| Protective activity of hamamelitannin on cell damage of murine skin fibroblasts induced by UVB irradiation. | |
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MedLine Citation:
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PMID: 7577835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protective activities of hamamelitannin (2',5-di-O-galloyl-hamamelose) in Hamamelis virginiana L. and its related compound, gallic acid, on damaged murine skin fibroblasts induced by UVB irradiation were investigated. In order to exclude the UV absorbing effect of the compounds, the protection study was performed such that the fibroblasts were pretreated with hamamelitannin or gallic acid for 24 h before UVB irradiation. At 200 microM concentration, hamamelitannin gave the higher survival of 72.6 +/- 0.4% in comparison with that of gallic acid (35.5 +/- 1.0%), while UVB absorbers such as 2-ethylhexyl p-methoxycinnamate and hexylbenzoate did not show such protection. The scavenging activities of hamamelitannin and gallic acid against active oxygens such as superoxide anion radicals, hydroxyl radicals and singlet oxygens were evaluated using electron spin resonance (ESR-spin trapping method). Hamamelitannin and gallic acid showed potent scavenging activities against all active oxygens tested. Furthermore, the association of hamamelitannin to fibroblasts was examined by comparing it with that of gallic acid, and the following results were obtained: (1) hamamelitannin reduces the reaction rate of liposome entrapped-nitroblue tetrazolium (NBT) with external superoxide anions, and (2) several glycosides associate with fibroblasts. From these results, it was concluded that hamamelitannin protects murine fibroblasts against external active oxygens by associating with the cell surface through its sugar moiety. |
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Authors:
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H Masaki; T Atsumi; H Sakurai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dermatological science Volume: 10 ISSN: 0923-1811 ISO Abbreviation: J. Dermatol. Sci. Publication Date: 1995 Jul |
Date Detail:
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Created Date: 1995-12-05 Completed Date: 1995-12-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9011485 Medline TA: J Dermatol Sci Country: IRELAND |
Other Details:
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Languages: eng Pagination: 25-34 Citation Subset: IM |
Affiliation:
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Shiga Central Laboratory, Noevir Co. Ltd., Youkaichi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Drug Carriers Fibroblasts / drug effects, radiation effects Free Radical Scavengers / pharmacology Gallic Acid / analogs & derivatives*, pharmacology Hexoses / pharmacology* Liposomes Mice Mice, Inbred ICR Nitroblue Tetrazolium / administration & dosage, pharmacology Radiation-Protective Agents / pharmacology* Reactive Oxygen Species / metabolism Skin / drug effects*, pathology, radiation effects* Superoxides / pharmacology Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Free Radical Scavengers; 0/Hexoses; 0/Liposomes; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 149-91-7/Gallic Acid; 298-83-9/Nitroblue Tetrazolium; 469-32-9/hamamelitannin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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