Document Detail

Protective activity of hamamelitannin on cell damage of murine skin fibroblasts induced by UVB irradiation.
MedLine Citation:
PMID:  7577835     Owner:  NLM     Status:  MEDLINE    
The protective activities of hamamelitannin (2',5-di-O-galloyl-hamamelose) in Hamamelis virginiana L. and its related compound, gallic acid, on damaged murine skin fibroblasts induced by UVB irradiation were investigated. In order to exclude the UV absorbing effect of the compounds, the protection study was performed such that the fibroblasts were pretreated with hamamelitannin or gallic acid for 24 h before UVB irradiation. At 200 microM concentration, hamamelitannin gave the higher survival of 72.6 +/- 0.4% in comparison with that of gallic acid (35.5 +/- 1.0%), while UVB absorbers such as 2-ethylhexyl p-methoxycinnamate and hexylbenzoate did not show such protection. The scavenging activities of hamamelitannin and gallic acid against active oxygens such as superoxide anion radicals, hydroxyl radicals and singlet oxygens were evaluated using electron spin resonance (ESR-spin trapping method). Hamamelitannin and gallic acid showed potent scavenging activities against all active oxygens tested. Furthermore, the association of hamamelitannin to fibroblasts was examined by comparing it with that of gallic acid, and the following results were obtained: (1) hamamelitannin reduces the reaction rate of liposome entrapped-nitroblue tetrazolium (NBT) with external superoxide anions, and (2) several glycosides associate with fibroblasts. From these results, it was concluded that hamamelitannin protects murine fibroblasts against external active oxygens by associating with the cell surface through its sugar moiety.
H Masaki; T Atsumi; H Sakurai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of dermatological science     Volume:  10     ISSN:  0923-1811     ISO Abbreviation:  J. Dermatol. Sci.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-12-05     Completed Date:  1995-12-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9011485     Medline TA:  J Dermatol Sci     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  25-34     Citation Subset:  IM    
Shiga Central Laboratory, Noevir Co. Ltd., Youkaichi, Japan.
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MeSH Terms
Drug Carriers
Fibroblasts / drug effects,  radiation effects
Free Radical Scavengers / pharmacology
Gallic Acid / analogs & derivatives*,  pharmacology
Hexoses / pharmacology*
Mice, Inbred ICR
Nitroblue Tetrazolium / administration & dosage,  pharmacology
Radiation-Protective Agents / pharmacology*
Reactive Oxygen Species / metabolism
Skin / drug effects*,  pathology,  radiation effects*
Superoxides / pharmacology
Ultraviolet Rays*
Reg. No./Substance:
0/Drug Carriers; 0/Free Radical Scavengers; 0/Hexoses; 0/Liposomes; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 149-91-7/Gallic Acid; 298-83-9/Nitroblue Tetrazolium; 469-32-9/hamamelitannin

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