| Protective Role of D-Amino Acid Oxidase Against Staphylococcus aureus infection. | |
| | |
MedLine Citation:
|
PMID: 22271930 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
D-amino acid oxidase (DAO) is a hydrogen peroxide-generating enzyme that uses a D-amino acid as a substrate. We hypothesized that DAO may protect against bacterial infection, because hydrogen peroxide is one of the most important molecules in the antibacterial defense systems in mammals. We show here that DAO suppressed the growth of Staphylococcus aureus in a manner that depended on the concentration of DAO and D-amino acid in vitro. Addition of catalase abolished the bacteriostatic activity of DAO. Although DAO plus D-Ala showed less bactericidal activity, addition of MPO greatly enhanced the bactericidal activity of DAO. Furthermore, DAO was able to utilize bacterial lysate, which contains D-Ala derived from peptidoglycan, which could produce hydrogen peroxide, with the consequence being, in the presence of myeloperoxidase, formation of hypochlorous acid. This concerted reaction of DAO and MPO led to the bactericidal action. In vivo experiments showed that DAO(-/-) (mutant) mice were more susceptible to S. aureus infection than were DAO(+/+) (wild-type) mice. These results suggest that DAO, together with myeloperoxidase, may serve an important role in antibacterial systems in mammals. |
| | |
Authors:
|
Hideaki Nakamura; Jun Fang; Hiroshi Maeda |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-23 |
Journal Detail:
|
Title: Infection and immunity Volume: - ISSN: 1098-5522 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Science, Sojo University, Ikeda 4-22-1, Kumamoto, Japan 860-0082. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cumulative parenthood rates in 1735 couples: impact of male factor infertility.
Next Document: A thermodynamical model for stress-fiber organization in contractile cells.