Document Detail


Protective effects of rutin on rat glomerular mesangial cells cultured in high glucose conditions.
MedLine Citation:
PMID:  21425374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The protective effect of rutin on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells was investigated. The cultured mesangial cells were divided into eight groups: normal, solvent control, high glucose, low dose of rutin, moderate dose of rutin, high dose of rutin, captopril and Ginkgo biloba extract. The cell cycles, type IV collagen and laminin in cytoplasm, TGF-β₁ mRNA of mesangial cells, Smad 2/3 and Smad 7, and the activities of four antioxidant indexes including T-SOD, MDA, CAT and GSH-Px were measured by flow cytometry, radioimmunoassay, RT-PCR, western blotting and visible spectrophotometry, respectively. Compared with the high glucose group, rutin decreased the cell percentages of the G₀/G₁ phase and inhibited the expression of Smad 2/3, laminin and type IV collagen, and TGF-β₁ mRNA level, significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression were significantly increased by rutin. These results suggest that rutin is a potent protective agent against glomerulosclerosis in DN.
Authors:
Dao-quan Tang; Ya-qin Wei; Yuan-yuan Gao; Xiao-xing Yin; Dong-zhi Yang; Jie Mou; Xiang-lan Jiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication     Date:  2011-03-21
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  25     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-24     Completed Date:  2012-02-22     Revised Date:  2013-11-25    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1640-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism
Captopril / pharmacology
Cell Cycle / drug effects
Cells, Cultured
Collagen Type IV / metabolism
Culture Media / chemistry
Diabetic Nephropathies / prevention & control
Glucose / pharmacology*
Laminin / metabolism
Mesangial Cells / drug effects*
Oxidative Stress
Protective Agents / pharmacology*
Rats
Rutin / pharmacology*
Smad2 Protein / metabolism
Smad3 Protein / metabolism
Smad7 Protein / metabolism
Transforming Growth Factor beta1 / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Collagen Type IV; 0/Culture Media; 0/Laminin; 0/Madh2 protein, rat; 0/Madh3 protein, rat; 0/Protective Agents; 0/Smad2 Protein; 0/Smad3 Protein; 0/Smad7 Protein; 0/Smad7 protein, rat; 0/Transforming Growth Factor beta1; 5G06TVY3R7/Rutin; 9G64RSX1XD/Captopril; IY9XDZ35W2/Glucose
Comments/Corrections
Retraction In:
Phytother Res. 2013 Oct;27(10):1580   [PMID:  24244948 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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