Document Detail


Protective effects of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via antioxidant mechanisms and Ca(2+) antagonism.
MedLine Citation:
PMID:  20577899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Preclinical and clinical investigations have shown hippocampal neuronal atrophy and destruction were observed in patients with depression, which could be ameliorated by the treatment with antidepressants. Therefore, neuroprotection has been proposed to be one of the acting mechanisms of antidepressant. Paeoniflorin, a monoterpene glycoside, has been reported to display antidepressant-like effects in animal models of behavioral despair. The present study aimed to examine the protective effect of paeoniflorin on glutamate-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The results showed that pretreatment with paeoniflorin elevated cell viability, inhibited apoptosis, decreased levels of intracellular reactive oxygen species and malondialdehyde, and enhanced activity of superoxide dismutase in glutamate-treated PC12 cells. Pretreatment with paeoniflorin also reversed the increased intracellular Ca(2+) concentration and the reduced Calbindin-D28K mRNA level caused by glutamate in PC12 cells. The results suggest that paeoniflorin exerts a neuroprotective effect on glutamate-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and Ca(2+) overload. This neuroprotective effect may be one of the action pathways accounting for the in vivo antidepressant activity of paeoniflorin.
Authors:
Qing-Qiu Mao; Xiao-Ming Zhong; Chun-Rong Feng; Ai-Juan Pan; Zhao-Yi Li; Zhen Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-25
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  30     ISSN:  1573-6830     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1059-66     Citation Subset:  IM    
Affiliation:
School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China. hzm1001@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Antidepressive Agents / pharmacology
Antioxidants / chemistry,  pharmacology*
Benzoic Acids / chemistry,  pharmacology*
Bridged Compounds / chemistry,  pharmacology*
Calcium / metabolism*
Calcium-Binding Protein, Vitamin D-Dependent / metabolism
Cell Survival / drug effects
Glucosides / chemistry,  pharmacology*
Glutamic Acid / toxicity*
Humans
Malondialdehyde / metabolism
Molecular Structure
Neuroprotective Agents / chemistry,  pharmacology*
Neurotoxicity Syndromes
Oxidative Stress / drug effects
PC12 Cells
Rats
Reactive Oxygen Species / metabolism
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antidepressive Agents; 0/Antioxidants; 0/Benzoic Acids; 0/Bridged Compounds; 0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Glucosides; 0/Neuroprotective Agents; 0/Reactive Oxygen Species; 0/calbindin; 23180-57-6/peoniflorin; 542-78-9/Malondialdehyde; 56-86-0/Glutamic Acid; 7440-70-2/Calcium; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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