Document Detail


Protective effect of thymoquinone in experimental testicular torsion.
MedLine Citation:
PMID:  20628240     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVES: To investigate the protective role of thymoquinone (TQ) on unilateral testicular ischemia-reperfusion (I/R) injury in mice.
MATERIALS AND METHODS: Experiments were performed on male C57BL/6 mice (8 weeks old, 20-25 g). The animals were divided into 3 groups including 6 mice in each group: control (sham), torsion/detorsion (TD) and TD+TQ. Mice, except the sham-operated group, were subjected to left unilateral torsion (720° rotation in the clockwise direction). The experiments were finished after sham operation time for controls, 120 min torsion and 240 min detorsion for the other groups. In the TD+TQ group 10 mg TQ was injected intraperitoneally 30 min before detorsion.
RESULTS: In the TD group total oxidative stress (TOS), oxidative stress index (OSI) and malondialdehyde (MDA) levels were higher than in the controls. TQ treatment decreased MDA, TOS and OSI values, but did not affect the total antioxidant capacity and myeloperoxidase activity in the TD+TQ group. Upon histological examination, mice in the TD group displayed moderate-to-severe disruption of the seminiferous epithelium. Treatment with TQ resulted in significantly reduced histological damage associated with I/R injury.
CONCLUSION: Our results suggested that TQ treatment may have a protective effect on testicular I/R injury.
Authors:
Ahmet Gökçe; Suleyman Oktar; Ahmet Koc; Ramazan Gonenci; Fatih Yalcinkaya; Zafer Yonden; Mehmet Duru
Publication Detail:
Type:  Journal Article     Date:  2010-07-13
Journal Detail:
Title:  Urologia internationalis     Volume:  85     ISSN:  1423-0399     ISO Abbreviation:  Urol. Int.     Publication Date:  2010  
Date Detail:
Created Date:  2010-12-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417373     Medline TA:  Urol Int     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  461-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 S. Karger AG, Basel.
Affiliation:
Department of Urology, Mustafa Kemal University, Tayfur Ata Sökmen Medical School, Antakya, Turkey. aagokce@yahoo.com
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