| Protective effect of PACAP against doxorubicin-induced cell death in cardiomyocyte culture. | |
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MedLine Citation:
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PMID: 20405239 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Pituitary adenylate cyclase-activating polypeptide (PACAP) is a widely distributed endogenous neuropeptide, also occurring in the cardiovascular system. Among others, PACAP has been suggested as a cardioprotective factor. It has been shown that PACAP inhibits cardiac fibrosis and protects cardiomyocytes against oxidative stress and in vitro ischemia/reperfusion. The aim of the present study was to investigate whether PACAP is protective in doxorubicin-induced cell death of cardiomyocytes. Cardiomyocytes were exposed to 1 µM doxorubicin for 24 h, which resulted in a marked reduction of cell viability and a parallel increase of apoptotic cells assessed by MTT test and annexin V/propidium iodide flow cytometry assay. Co-incubation with 20 nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, doxorubicin increased the activation of caspase-3 and decreased the phosphorylation of Bad, while simultaneous PACAP treatment reduced the caspase-3 activation and increased the level of phospho-Bad. In summary, our present results demonstrate that PACAP effectively protects cardiomyocytes against doxorubicin-induced apoptotic cell death. |
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Authors:
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Boglarka Racz; Dora Reglodi; Gabriella Horvath; Andras Szigeti; Borbala Balatonyi; Erzsebet Roth; Gyorgy Weber; Nasri Alotti; Gabor Toth; Balazs Gasz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-20 |
Journal Detail:
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Title: Journal of molecular neuroscience : MN Volume: 42 ISSN: 1559-1166 ISO Abbreviation: J. Mol. Neurosci. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002991 Medline TA: J Mol Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 419-27 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Chemistry, University of Pecs, Szigeti u 12, Pecs, Hungary. boglarka.racz@aok.pte.hu |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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