Document Detail


Protective effect of PACAP against doxorubicin-induced cell death in cardiomyocyte culture.
MedLine Citation:
PMID:  20405239     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a widely distributed endogenous neuropeptide, also occurring in the cardiovascular system. Among others, PACAP has been suggested as a cardioprotective factor. It has been shown that PACAP inhibits cardiac fibrosis and protects cardiomyocytes against oxidative stress and in vitro ischemia/reperfusion. The aim of the present study was to investigate whether PACAP is protective in doxorubicin-induced cell death of cardiomyocytes. Cardiomyocytes were exposed to 1 µM doxorubicin for 24 h, which resulted in a marked reduction of cell viability and a parallel increase of apoptotic cells assessed by MTT test and annexin V/propidium iodide flow cytometry assay. Co-incubation with 20 nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, doxorubicin increased the activation of caspase-3 and decreased the phosphorylation of Bad, while simultaneous PACAP treatment reduced the caspase-3 activation and increased the level of phospho-Bad. In summary, our present results demonstrate that PACAP effectively protects cardiomyocytes against doxorubicin-induced apoptotic cell death.
Authors:
Boglarka Racz; Dora Reglodi; Gabriella Horvath; Andras Szigeti; Borbala Balatonyi; Erzsebet Roth; Gyorgy Weber; Nasri Alotti; Gabor Toth; Balazs Gasz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-20
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  42     ISSN:  1559-1166     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  419-27     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Medical Chemistry, University of Pecs, Szigeti u 12, Pecs, Hungary. boglarka.racz@aok.pte.hu
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