| The protective effect of ischemic preconditioning associated with altered gene expression profiles in rat lung after reperfusion. | |
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MedLine Citation:
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PMID: 20097364 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Ischemia-reperfusion (IR) injury remains an important clinical problem after lung transplantation. It is well-known that ischemic preconditioning (IPC) markedly enhances the ability of organs to withstand a sustained ischemia and reperfusion injury. But the molecular mechanisms of this protective effect are poorly understood. Therefore, we used gene microarrays to profile gene expression patterns involved in ischemia precondition. METHODS: Three groups of rats were studied: IR group (IR 0, 1, 3, 6, 24, n = 5 each group, IPC group (IP 1, IP 3, 6, 24h, n = 5 each group) and Sham group (n = 5). Samples were collected from different groups. The RatRef-12 expression Beadchip (22,226 gene probes per array) was used to analyze the pattern of gene expression in all groups. RESULTS: Microarray analysis showed that 648, 340, 711, 1279, and 641 genes were differentially expressed in IR 0-, 1-, 3-, 6- and 24-h groups, respectively. The profile revealed that IPC induced significant alterations of expression of many genes encoding inflammatory associated factors, oxidation and antioxidant molecules, apoptosis regulatory protein, metabolic enzyme, and ion channel protein. CONCLUSIONS: The damage and repair after lung IR are a dynamic process. IPC affects gene expression profiles in IR lung tissue mainly within 6h, and sustains until 24h later. IPC reduces lung IR injury mainly through anti-inflammatory response, antioxidative stress, and regulating cell energy metabolism and ion channels. |
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Authors:
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Nie Jun; Jiang Ke; Cheng Gang; Cheng Lin; Li Jinsong; Wang Jianjun |
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Publication Detail:
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Type: Journal Article Date: 2009-11-10 |
Journal Detail:
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Title: The Journal of surgical research Volume: 168 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 281-93 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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