| Protective Effect of Grape Seed Extract against Oxidative Stress-Induced Cell Death in a Staurosporine-Differentiated Retinal Ganglion Cell Line. | |
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MedLine Citation:
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PMID: 22440165 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective: Grape seed extract (GSE) is a potent antioxidant. We examined the effect of GSE on oxidative stress-induced cell death in a transformed retinal ganglion cell line, RGC-5. Methods: Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with 1 µM staurosporine were incubated with GSE for 2 h and then exposed to buthionine sulfoximine plus glutamate (B/G) for 24 h. Cell death was detected using the LIVE/DEAD viability assay and the type of cell death was evaluated using fluorescein isothiocyanate-conjugated Annexin-V/propidium iodide staining. To investigate the mechanism underlying cell death, we determined the caspase-3 activity and level of reactive oxygen species (ROS) formation. Results: Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis (not necrosis) with increasing caspase-3 activity. GSE rescued the ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting both intracellular ROS production and caspase-3 activation. Conclusion: GSE had a neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells. |
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Authors:
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Hongseok Yang; Bo Kyung Lee; Koung Hoon Kook; Yi-Sook Jung; Jaehong Ahn |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Current eye research Volume: 37 ISSN: 1460-2202 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-3-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: - |
Other Details:
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Languages: ENG Pagination: 339-344 Citation Subset: - |
Affiliation:
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Department of Ophthalmology, Ajou University School of Medicine , Suwon , Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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