Document Detail


Protective Effect of Grape Seed Extract against Oxidative Stress-Induced Cell Death in a Staurosporine-Differentiated Retinal Ganglion Cell Line.
MedLine Citation:
PMID:  22440165     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective: Grape seed extract (GSE) is a potent antioxidant. We examined the effect of GSE on oxidative stress-induced cell death in a transformed retinal ganglion cell line, RGC-5. Methods: Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with 1 µM staurosporine were incubated with GSE for 2 h and then exposed to buthionine sulfoximine plus glutamate (B/G) for 24 h. Cell death was detected using the LIVE/DEAD viability assay and the type of cell death was evaluated using fluorescein isothiocyanate-conjugated Annexin-V/propidium iodide staining. To investigate the mechanism underlying cell death, we determined the caspase-3 activity and level of reactive oxygen species (ROS) formation. Results: Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis (not necrosis) with increasing caspase-3 activity. GSE rescued the ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting both intracellular ROS production and caspase-3 activation. Conclusion: GSE had a neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells.
Authors:
Hongseok Yang; Bo Kyung Lee; Koung Hoon Kook; Yi-Sook Jung; Jaehong Ahn
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Current eye research     Volume:  37     ISSN:  1460-2202     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-3-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  339-344     Citation Subset:  -    
Affiliation:
Department of Ophthalmology, Ajou University School of Medicine , Suwon , Republic of Korea.
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