| Protection of spermatogenesis against cytotoxic effects of two chemotherapeutic drugs by temporary testicular blood flow interruption in the ram. | |
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MedLine Citation:
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PMID: 8250288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Temporary interruption of the testicular blood flow for 1 h after injection of cytostatic drugs has a protective effect on spermatogenesis. This was shown in experiments in which spermatogenesis was evaluated at four weeks after a single intravenous injection of Adriblastina (ADR; doxorubicin hydrochloride) or Mitomycin-C-kyowa (MIT). Interruption of the blood flow was performed by inflation of an occluder implanted around the testicular artery. The animals were killed and histological sections prepared 4 weeks after treatment. In all drug-treated animals spermatids were near absence and spermatocytes were decreased in number. Therefore, even after occlusion of the blood flow, the drug doses were high enough to kill not only large numbers of differentiating spermatogonia but also stem cells. The response of the stem cells to the treatments was evaluated by counting the numbers of A spermatogonia per 100 Sertoli cells in the different groups. Normal numbers of these cells were found after both MIT and ADR, indicating that the stem cell population had responded to the initial cell loss by extra proliferation. However, significantly higher numbers of A spermatogonia were found in the drug-treated animals in which the testicular blood flow was interrupted for 1 h. This indicates that occlusion of the blood flow to the testis for 1 h results in a faster recovery of spermatogenesis than after drug treatment alone. |
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Authors:
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J van Vliet; D G de Rooij; C J Wensing; A L Bootsma |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Andrologia Volume: 25 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 1993 Sep-Oct |
Date Detail:
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Created Date: 1993-12-29 Completed Date: 1993-12-29 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: GERMANY |
Other Details:
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Languages: eng Pagination: 251-6 Citation Subset: IM |
Affiliation:
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Department of Anatomy, School of Veterinary Medicine, State University, Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Constriction Doxorubicin / toxicity* Evaluation Studies as Topic Ischemia / pathology Male Mitomycin / toxicity* Oligospermia / chemically induced, prevention & control Regional Blood Flow Seminiferous Epithelium / drug effects, pathology Sheep Spermatogenesis / drug effects* Testis / blood supply*, drug effects, pathology |
| Chemical | |
Reg. No./Substance:
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23214-92-8/Doxorubicin; 50-07-7/Mitomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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