Document Detail


Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol.
MedLine Citation:
PMID:  15977073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 mug GTP ml(-1) significantly (p<0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.
Authors:
Dong-Wook Han; Hak Hee Kim; Mi Hee Lee; Hyun Sook Baek; Kwon-Yong Lee; Suong-Hyu Hyon; Jong-Chul Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  27     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-24     Completed Date:  2005-10-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  655-60     Citation Subset:  IM    
Affiliation:
Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Cell Line, Tumor
Cell Proliferation
Cryopreservation
Dimethyl Sulfoxide / chemistry
Flavonoids / chemistry,  metabolism
Freezing
Guanosine Triphosphate / metabolism
Humans
Osteoblasts / cytology*,  metabolism
Oxidative Stress
Phenols / chemistry,  metabolism
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
Tea
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Phenols; 0/Reactive Oxygen Species; 0/Tea; 0/polyphenols; 67-68-5/Dimethyl Sulfoxide; 86-01-1/Guanosine Triphosphate; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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