| Protection of oligonucleotides against enzymatic degradation by pegylated and nonpegylated branched polyethyleneimine. | |
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MedLine Citation:
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PMID: 17358077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Among the cationic polymers, polyethyleneimine (PEI) is a promising candidate for delivery of oligodeoxynucleotides (ODNs). In this study, we wondered whether pegylation of PEI influences the complexation with ODNs. We especially aimed to investigate whether ODNs are differently protected against enzymatic degradation in PEI and polyethylene glycol-polyethyleneimine (PEG-PEI) polyplexes. Using fluorescence resonance energy transfer combined with fluorescence correlation spectroscopy, we found that PEI/ODN polyplexes remain to protect the ODNs they carry over a prolonged period of time while in PEG-PEI/ODN polyplexes the degradation of the ODNs slowly proceeds. We attribute this to the fact that PEI seems to compact the ODNs more firmly in the polyplexes' core than PEG-PEI, which apparently also results in a better protection against enzymatic degradation. These observations may also influence the efficiency of PEI-based ODN delivery in vivo, where pegylation is an attractive strategy to enhance the stability of the polyplexes in the blood stream. |
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Authors:
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K Remaut; B Lucas; K Raemdonck; K Braeckmans; J Demeester; S C De Smedt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-15 |
Journal Detail:
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Title: Biomacromolecules Volume: 8 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-09 Completed Date: 2007-09-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 1333-40 Citation Subset: IM |
Affiliation:
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Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Deoxyribonuclease I
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chemistry* Drug Carriers / chemistry* Fluorescence Resonance Energy Transfer / methods Molecular Structure Oligonucleotides / chemistry* Particle Size Polyethyleneimine / chemistry* Sensitivity and Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Oligonucleotides; 9002-98-6/Polyethyleneimine; EC 3.1.21.1/Deoxyribonuclease I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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