Document Detail

Protection of neuro-2a cells against calcium ionophore cytotoxicity by gangliosides.
MedLine Citation:
PMID:  1573675     Owner:  NLM     Status:  MEDLINE    
Gangliosides are known to assert both neuritogenic and neuroprotective effects when applied to a variety of neuroblastoma and primary neuronal cultures. We have developed a model employing Neuro-2a neuroblastoma cells with Ca2+ ionophore A23187 as neurotoxic agent causing neurite retraction and eventual cell death. Gangliosides attenuated the toxicity of this substance, increasing both cell survival and neurite stability. In one series of experiments, cells were exposed to A23187 for 24 hr and then incubated in fresh medium (washout) for 18 hr; gangliosides were present at varying times. The paradigm in which cells were only preincubated (2 hr) with ganglioside provided no benefit, nor did incubation of the cells in both ionophore and ganglioside during the 24-hr exposure period. Significant protection was achieved by exposing the cells to ganglioside after washout of A23187, or continuously throughout the whole period. Bovine brain ganglioside mixture and the four major components (GM1, GD1a, GD1b, GT1b) applied individually were all effective. By contrast, GM3 and GM1-alcohol, a neutral derivative of GM1, provided little or no protection. Dichlorobenzamil, an inhibitor of the Na(+)-Ca2+ exchanger, tended to block the neurite stabilizing effect of gangliosides, suggesting that the mechanism might involve potentiation of this antiporter.
K Nakamura; G Wu; R W Ledeen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  31     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-06-04     Completed Date:  1992-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  245-53     Citation Subset:  IM    
Albert Einstein College of Medicine, Department of Neurology, Bronx, New York.
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MeSH Terms
Brain Chemistry / drug effects
Calcimycin / antagonists & inhibitors*,  toxicity
Calcium / physiology*
Cell Survival / drug effects
Cells, Cultured
Gangliosides / pharmacology*
Ion Exchange
Neurites / drug effects
Neuroblastoma / physiopathology
Neurons / drug effects*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Gangliosides; 52665-69-7/Calcimycin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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