Document Detail


Protection of ischemic hippocampal neurons by ginsenoside Rb1, a main ingredient of ginseng root.
MedLine Citation:
PMID:  9237267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our previous study showed that the oral administration of red ginseng powder before but not after transient forebrain ischemia prevented delayed neuronal death in gerbils, and that a neuroprotective molecule within red ginseng powder was ginsenoside Rb1. However, it remains to be clarified whether or not ginsenoside Rb1 acts directly on the ischemic brain, and the mechanism by which ginsenoside Rb1 protects the ischemic CA1 neurons is not determined. Without elucidation of the pharmacological property of ginsenoside Rb1, the drug would not be accepted as a neuroprotective agent. The present study demonstrated that the intracerebroventricular infusion of ginsenoside Rb1 after 3.5 min or 3 min forebrain ischemia, precluded significantly the ischemia-induced shortening of response latency in a step-down passive avoidance task and rescued a significant number of hippocampal CA1 neurons from lethal ischemic damage. The intracerebroventricular infusion of ginsenoside Rb1 did not affect hippocampal blood flow or hippocampal temperature except that it caused a slight increase in hippocampal blood flow at 5 min after transient forebrain ischemia. Furthermore, ginsenoside Rb1 at concentrations of 0.1-100 fg/ml (0.09-90 fM) rescued hippocampal neurons from lethal damage caused by the hydroxyl radical-promoting agent FeSO4 in vitro, and the Fenton reaction system containing p-nitrosodimethylaniline confirmed the hydroxyl radical-scavenging activity of ginsenoside Rb1. These findings suggest that the central infusion of ginsenoside Rb1 after forebrain ischemia protects hippocampal CA1 neurons against lethal ischemic damage possibly by scavenging free radicals which are overproduced in situ after brain ischemia and reperfusion. The present study may validate the empirical usage of ginseng root over thousands of years for the prevention of cerebrovascular diseases.
Authors:
J H Lim; T C Wen; S Matsuda; J Tanaka; N Maeda; H Peng; J Aburaya; K Ishihara; M Sakanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  28     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-12     Completed Date:  1997-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  191-200     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Ehime University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Avoidance Learning / drug effects
Body Temperature / drug effects
Brain Ischemia / pathology*
Cell Death / drug effects
Central Nervous System Agents / administration & dosage,  pharmacology*
Cerebrovascular Circulation / drug effects
Female
Gerbillinae
Ginsenosides
Hippocampus / blood supply,  pathology*
Immunoblotting
Injections, Intraventricular
Male
Microtubule-Associated Proteins / metabolism
Neurons / drug effects*
Panax / chemistry*
Plants, Medicinal*
Prosencephalon / blood supply,  pathology
Saponins / administration & dosage,  pharmacology*
Chemical
Reg. No./Substance:
0/Central Nervous System Agents; 0/Ginsenosides; 0/Microtubule-Associated Proteins; 0/Saponins

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