| Protection of human corneal epithelial cells from hypoxia-induced disruption of barrier function by keratinocyte growth factor. | |
| | |
MedLine Citation:
|
PMID: 18362114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: The possible detrimental effect of hypoxia on the barrier function of corneal epithelial cells and whether keratinocyte growth factor (KGF) might protect against such an effect were investigated. METHODS: Simian virus 40-transformed human corneal epithelial (HCE) cells were cultured for 4 days to allow the establishment of barrier function. They were then deprived of serum for 24 hours before exposure to 1% (hypoxia) or 21% (normoxia) oxygen for 24 hours. Barrier function was evaluated by measurement of transepithelial electrical resistance (TER). The localization of ZO-1 and occludin was determined by immunofluorescence microscopy, and the expression of these tight junctional proteins as well as the phosphorylation of the mitogen-activated protein kinases ERK, p38, and JNK were examined by immunoblot analysis. RESULTS: Hypoxia induced a decrease in the TER of HCE cells compared with that of cells maintained under normoxia. The localization of ZO-1 at cell-cell borders was disrupted by hypoxia, whereas the distribution of occludin was not affected. Hypoxia also induced the downregulation of ZO-1 and a decrease in the phosphorylation of ERK without affecting the phosphorylation of p38 or JNK. All these effects of hypoxia were inhibited by KGF. The effects of KGF on TER and ZO-1 localization in cells exposed to hypoxia were inhibited by PD98059, an inhibitor of ERK signaling. Neither hypoxia nor KGF exhibited mitogenic or cytotoxic effects in HCE cells. CONCLUSIONS: Hypoxia induces disruption of the barrier function of HCE cells by eliciting the redistribution and degradation of ZO-1, and this effect is inhibited by KGF in a manner dependent on ERK activation. |
| | |
Authors:
|
Shinichiro Teranishi; Kazuhiro Kimura; Koji Kawamoto; Teruo Nishida |
Related Documents
:
|
3632684 - Fluoride effects on 31p nmr spectra of macrophages. 15840654 - Manipulation of redox signaling in mammalian cells enabled by controlled photogeneratio... 10498294 - Reactive oxygen species production by monoamine oxidases in intact cells. 9501444 - A fermentor system for regulating oxygen at low concentrations in cultures of saccharom... 10811124 - Photorepair prevents ultraviolet-induced apoptosis in human cells expressing the marsup... 20668294 - Induction of human beta-cell proliferation and engraftment using a single g1/s regulato... |
Publication Detail:
|
Type: Journal Article Date: 2008-03-24 |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 49 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-06-02 Completed Date: 2008-07-18 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 2432-7 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anoxia
/
metabolism* Cell Line, Transformed Cell Membrane Permeability / drug effects Cell Proliferation Cell Transformation, Viral Electric Impedance Epithelium, Corneal / drug effects*, metabolism* Fibroblast Growth Factor 7 / pharmacology* Humans Immunoblotting JNK Mitogen-Activated Protein Kinases / metabolism Membrane Proteins / metabolism Microscopy, Fluorescence Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Phosphoproteins / metabolism Phosphorylation Tight Junctions / drug effects p38 Mitogen-Activated Protein Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Membrane Proteins; 0/Phosphoproteins; 0/occludin; 0/zonula occludens-1 protein; 126469-10-1/Fibroblast Growth Factor 7; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Abnormal cellular reactivity to microbial antigens in patients with uveitis.
Next Document: Endovascular treatment of ileocolic pseudoaneurysm after a laparoscopic-assisted bowel resection for...