| Protection of hepatocytes from apoptosis by a novel substance from actinomycetes culture medium. | |
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MedLine Citation:
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PMID: 15806978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel substance, #675, found from an Streptomyces sp. SM675 culture medium, dose-dependently stimulates the proliferation of human functional liver cell 4 (FLC4). When FLC4 cells were incubated under conditions without fetal bovine serum (FBS), typical features of apoptotic cell death such as shrinkage and nuclear condensation appeared; high molecular weight (HMW) DNA fragments were found; and caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were cleaved. When FLC4 cells were incubated with #675 and without FBS, the cells grew healthy, no HMW DNA fragments were found, and caspase-3 and PARP cleavage weakened, suggesting that #675 protects FLC4 cells from apoptosis induced by FBS-deprivation. The quantitative reverse-transcribed polymerase chain reaction did not show differences in PARP or Bcl-2 mRNA expression in FLC4 cells incubated with or without #675, indicating other genes may be involved in this anti-apoptosis effect. These results show that #675 enhances FLC4 proliferation via an apoptosis-inhibition pathway, implying potential pharmacological and clinical applications. |
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Authors:
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Yang Liu; Saori Takahashi; Hironobu Ogasawara; Han Geuk Seo; Masami Kawagoe; Fujiko Hirasawa; Naxin Guo; Yasuharu Ueno; Takashi Kameda; Toshihiro Sugiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical research (Tokyo, Japan) Volume: 26 ISSN: 0388-6107 ISO Abbreviation: Biomed. Res. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-04-05 Completed Date: 2005-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100317 Medline TA: Biomed Res Country: Japan |
Other Details:
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Languages: eng Pagination: 9-14 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, School of Medicine, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Base Sequence Caspase 3 Caspases / metabolism Cell Line Culture Media DNA Fragmentation / drug effects DNA, Complementary / genetics Drug Evaluation, Preclinical Genes, bcl-2 / drug effects Hepatocytes / cytology*, drug effects*, metabolism Humans Japan Poly(ADP-ribose) Polymerases / genetics, metabolism RNA, Messenger / genetics, metabolism Soil Microbiology Streptomyces / chemistry*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA, Complementary; 0/RNA, Messenger; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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