Document Detail

Protection from oxidative stress by methionine sulfoxide reductases in RPE cells.
MedLine Citation:
PMID:  15993845     Owner:  NLM     Status:  MEDLINE    
We investigated the role of methionine sulfoxide reductases (Msrs) in oxidant-stress-induced cell death in retinal pigmented epithelial (RPE) cells. In RPE cells exposed to varying doses of H(2)O(2), gene expression of MsrA and hCBS-1 (the human analog of MsrB2) increased in a dose-dependent and time-dependent manner with maximal increase with 150 microM H(2)O(2) in 24h. H(2)O(2) treatment resulted in the generation of reactive oxygen species and activation of caspase 3. Confocal microscopic and protein analysis showed an increase in MsrA expression in cytosol and mitochondria. Silencing of MsrA resulted in caspase 3 induction and accentuated cell death from H(2)O(2). Focal, strong immunoreactivity for MsrA was observed in sub-RPE macular drusen from patients with age-related macular degeneration. In summary, our data show that MsrA and hCBS-1 are up-regulated in oxidative stress to counteract injury to RPE.
Parameswaran G Sreekumar; Ram Kannan; Jennifer Yaung; Christine K Spee; Stephen J Ryan; David R Hinton
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  334     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-15     Completed Date:  2005-09-15     Revised Date:  2012-05-28    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-53     Citation Subset:  IM    
Arnold and Mabel Beckman Macular Research Center, Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90033, USA.
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MeSH Terms
Aged, 80 and over
Apoptosis / drug effects
Cells, Cultured
Cytoprotection / drug effects*
Dose-Response Relationship, Drug
Hydrogen Peroxide / administration & dosage*
Macular Degeneration / metabolism*
Methionine Sulfoxide Reductases
Mitochondria / drug effects,  metabolism
Oxidative Stress / drug effects*
Oxidoreductases / metabolism*
Pigment Epithelium of Eye / drug effects,  metabolism*
Reactive Oxygen Species / metabolism*
Transcription Factors / metabolism*
Grant Support
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Transcription Factors; 7722-84-1/Hydrogen Peroxide; EC 1.-/Oxidoreductases; EC 1.8.4.-/MSRB2 protein, human; EC 1.8.4.-/Methionine Sulfoxide Reductases; EC sulfoxide reductase
Erratum In:
Biochem Biophys Res Commun. 2005 Dec 2;337(4):1353

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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