Document Detail

Protection from nitrosative stress by yeast flavohemoglobin.
MedLine Citation:
PMID:  10758168     Owner:  NLM     Status:  MEDLINE    
Yeast hemoglobin was discovered close to half a century ago, but its function has remained unknown. Herein, we report that this flavohemoglobin protects Saccharomyces cerevisiae from nitrosative stress. Deletion of the flavohemoglobin gene (YHB1) abolished the nitric oxide (NO)-consuming activity of yeast cells. Levels of protein nitrosylation were more than 10-fold higher in yhb1 mutant yeast than in isogenic wild-type cells after incubation with NO donors. Growth of mutant cells was inhibited by a nitrosative challenge that had little effect on wild-type cells, whereas the resistance of mutant cells to oxidative stress was unimpaired. Protection conferred by yeast flavohemoglobin against NO and S-nitrosothiols was seen under both anaerobic and aerobic conditions, consistent with a primary function in NO detoxification. A phylogenetic analysis indicated that protection from nitrosative stress is likely to be a conserved function among microorganismal flavohemoglobins. Flavohemoglobin is therefore a potential target for antimicrobial therapy.
L Liu; M Zeng; A Hausladen; J Heitman; J S Stamler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  97     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-24     Completed Date:  2000-05-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4672-6     Citation Subset:  IM    
Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Fungal Proteins / genetics,  metabolism
Gene Deletion
Hemeproteins / genetics,  metabolism*
Nitric Oxide / metabolism*
Oxidative Stress / physiology*
Saccharomyces cerevisiae / genetics,  metabolism,  physiology*
Saccharomyces cerevisiae Proteins*
Reg. No./Substance:
0/Fungal Proteins; 0/Hemeproteins; 0/Saccharomyces cerevisiae Proteins; 10102-43-9/Nitric Oxide; EC 1.13.11.-/Dioxygenases; EC protein, S cerevisiae

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