| Protection from C. difficile Toxin B-catalysed Rac/Cdc42 glucosylation by tauroursodeoxycholic acid-induced Rac/Cdc42 phosphorylation. | |
| | |
MedLine Citation:
|
PMID: 22059737 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Abstract Toxin A (TcdA) and Toxin B (TcdB) are the major virulence factors of Clostridium difficile-associated diarrhea (CDAD). TcdA and TcdB mono-glucoslyate small GTPases of the Rho family, thereby causing actin re-organisation in colonocytes, resulting in the loss of colonic barrier function. The hydrophilic bile acid tauroursodeoxycholic acid (TUDCA) is an approved drug for the treatment of cholestasis and biliary cirrhosis. In this study, TUDCAinduced activation of Akt 1 is presented to increase cellular levels of pS71-Rac1/Cdc42 in human hepatocarcinoma (Hep-G2) cells, showing for the first time that bile acid signalling affects the activity of Rho proteins. Rac1/Cdc42 phosphorylation, in turn, protects Rac1/Cdc42 from TcdB-catalysed glucosylation and reduces the TcdB-induced cytopathic effects in Hep-G2 cells. The results of this study indicate that TUDCA may prove useful as a therapeutic agent for the treatment of CDAD. |
| | |
Authors:
|
Vanessa Brandes; Ilona Schelle; Sophie Brinkmann; Florian Schulz; Janett Schwarz; Ralf Gerhard; Harald Genth |
Related Documents
:
|
7108527 - Effects of kainic acid on high-energy metabolites in the mouse striatum. 22539047 - A facile route to boronic acid functional polymeric microspheres via epoxide ring opening. 20493097 - Synaptosomal release of newly-synthetized or recently accumulated amino acids. differen... 9379437 - Structure--activity studies for alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropanoic aci... 14507447 - Iontophoresis promotes percutaneous absorption of l-ascorbic acid in rat skin. 15213987 - Separation of flavonoids and phenolic acids from propolis by capillary zone electrophor... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-7 |
Journal Detail:
|
Title: Biological chemistry Volume: - ISSN: 1437-4315 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institut für Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Initial therapy with the fixed-dose combination of sitagliptin and metformin results in greater impr...
Next Document: Protein conjugated with aldehydes derived from lipid peroxidation as an independent parameter of the...