Document Detail


Protection of the chronic hypoxic immature rat heart during global ischemia.
MedLine Citation:
PMID:  7887715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The benefit of cardioplegic cardiac arrest for the protection of immature myocardium is controversial. We therefore investigated the efficacy of (1) topical hypothermia alone, (2) slow cooling by coronary perfusion hypothermia, and (3) cardioplegic cardiac arrest for the protection of isolated immature rats hearts (28 days) during 8 hours of global ischemia at 10 degrees C. The study was conducted in hearts from rats that were kept hypoxemic by lifelong exposure to simulated high altitude. Left ventricular function, endothelial function, the metabolic status, and the extent of myocardial injury were all assessed. Topical hypothermia provided superior protection in hypoxic hearts, with recovery of the maximum developed left ventricular pressure by 70.6% +/- 18.0% (mean +/- standard deviation) of its preischemic value (p < 0.01 versus slow cooling and versus cardioplegic protection). The same pattern of recovery was observed among control hearts. The degree of recovery of endothelial function after sole topical hypothermia measured 54% +/- 36% in hypoxic hearts and 62% +/- 37% in control hearts, but was not recordable in any of the other groups. Creatine kinase leakage and the myocardial high-energy content did not differ significantly among any of the groups. Rapid cooling by topical hypothermia alone provides superior protection in chronic hypoxic, immature rat hearts versus the protection conferred by slow cooling. St. Thomas' Hospital cardioplegic solution II does not afford additional protection. Endothelial injury caused by cold asanguineous perfusates, including cardioplegia, interferes with the recovery of vascular function, which, in turn, may limit mechanical function.
Authors:
M Karck; G Ziemer; M Zoeller; S Schulte; K D Juergens; H Weisser; A Haverich
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  59     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-04-07     Completed Date:  1995-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  699-706     Citation Subset:  AIM; IM    
Affiliation:
Department of Cardiovascular Surgery, University of Kiel, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Bicarbonates / pharmacology
Blood Gas Analysis
Calcium Chloride / pharmacology
Cardioplegic Solutions / pharmacology
Cell Hypoxia
Chronic Disease
Creatine Kinase / metabolism
Endothelium, Vascular
Fetal Heart / drug effects,  metabolism,  physiopathology
Heart Arrest, Induced / methods*
Heart Function Tests
Hypothermia, Induced
Magnesium / pharmacology
Male
Myocardial Ischemia / etiology,  metabolism,  physiopathology,  prevention & control*
Myocardial Reperfusion Injury / etiology,  metabolism,  physiopathology,  prevention & control*
Oxygen Consumption
Phosphocreatine / metabolism
Potassium Chloride / pharmacology
Rats
Rats, Wistar
Sodium Chloride / pharmacology
Time Factors
Ventricular Function, Left
Chemical
Reg. No./Substance:
0/Bicarbonates; 0/Cardioplegic Solutions; 0/St. Thomas' Hospital cardioplegic solution; 10043-52-4/Calcium Chloride; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine; 7439-95-4/Magnesium; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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