| Protection of chickens from infectious bronchitis by in ovo and intramuscular vaccination with a DNA vaccine expressing the S1 glycoprotein. | |
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MedLine Citation:
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PMID: 12887187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have constructed a DNA vaccine (pDKArkS1-DPI) expressing the S1 glycoprotein (Arkansas DPI) of infectious bronchitis virus (IBV) to examine protective immunity after in ovo and intramuscular DNA immunization. Birds receiving in ovo DNA followed by live virus vaccination at 2 wk of age were 100% protected from clinical disease. Birds receiving only live virus vaccine or only in ovo DNA vaccination were < or = 80% protected. IBV was detected up to 10 days postchallenge in unvaccinated control groups, whereas birds receiving in ovo DNA and live virus vaccination cleared IBV from tracheal samples before day 5 postchallenge. Transcription of the S1 gene was confirmed in lung tissue after in ovo vaccination by an antisense riboprobe, and the S1 protein was detected by immunohistology in the heart and bursa. In a separate experiment, birds were injected intramuscularly with either 50, 100, or 150 microg of the DNA vaccine at 1 day of age and then again with either 100, 200, or 300 microg of the DNA vaccine, respectively, at 14 days of age. At 10 days postchallenge, no clinical signs were observed and no challenge virus was reisolated from the birds vaccinated with 150 microg and 300 microg of DNA. Between DNA-vaccinated birds and nonvaccinated control birds, no statistical differences were observed for IBV-specific serum antibodies as detected by enzyme-linked immunosorbent assay or the virus neutralization test. These data indicate that DNA vaccination with the S1 gene either in ovo or intramuscularly can provide birds with some protection against clinical disease after homologous IBV challenge. |
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Authors:
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Darrell R Kapczynski; Deborah A Hilt; David Shapiro; Holly S Sellers; Mark W Jackwood |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Avian diseases Volume: 47 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 2003 Apr-Jun |
Date Detail:
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Created Date: 2003-07-30 Completed Date: 2003-09-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: United States |
Other Details:
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Languages: eng Pagination: 272-85 Citation Subset: IM |
Affiliation:
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University of Georgia, Poultry Diagnostic and Research Center, College of Veterinary Medicine, 953 College Station Road, Athens, GA 30602, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / blood, immunology COS Cells Chickens / immunology, virology Coronavirus Infections / immunology, prevention & control, veterinary Drug Administration Routes Infectious bronchitis virus / immunology* Ovum / immunology Poultry Diseases / immunology*, prevention & control* Trachea / pathology, virology Vaccination / veterinary Vaccines, DNA / administration & dosage*, immunology* Viral Proteins / genetics Viral Vaccines / administration & dosage*, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Vaccines, DNA; 0/Viral Proteins; 0/Viral Vaccines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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