Document Detail


Protection against lipid peroxidation induced during preservation of lungs for transplantation.
MedLine Citation:
PMID:  7865534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The questions of whether oxygen-derived free radicals are induced during preservation of lungs and, if so, how such radicals might relate to reperfusion injury were investigated by means of an isolated canine lung model. Lungs were obtained from 16 mongrel dogs and divided into groups 1 (n = 6), 2 (n = 5), and 3 (n = 5). The lungs of groups 1, 2, and 3 were flushed through the pulmonary artery with Euro-Collins solution alone, the solution with superoxide dismutase (120,000 U/L), and the solution with allopurinol (1 mmol/L), respectively, at 4 degrees C and then stored for 4 hours in the respective solutions at 4 degrees C with clamped bronchi. They were then reperfused for 2 hours by means of an isolated lung model. Lung lipid peroxidation was sequentially determined. The lung functional status was assessed by systolic pulmonary arterial pressure and end-inspiratory airway pressure. The lung edema was assessed by lung wet/dry weight ratio. Lipid peroxidation was induced after 1 hour of preservation and the first 30 minutes of the reperfusion in group 1 and only 2 hours of the reperfusion in group 2, whereas no induction was observed in group 3. Values for systolic pulmonary arterial pressure and end-inspiratory pressure in group 1 were significantly higher than those in group 3 (p < 0.05). The lung wet/dry weight ratio in group 1 was significantly higher than that in groups 2 and 3 (p < 0.05). The present results indicate that the administration of free radical scavengers in the preservation may effectively improve conditions for lung transplantation.
Authors:
K Nezu; K Kushibe; T Tojo; N Sawabata; K Kawachi; Y Mizumoto; D Nakae; Y Konishi; S Kitamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation     Volume:  13     ISSN:  1053-2498     ISO Abbreviation:  J. Heart Lung Transplant.     Publication Date:    1994 Nov-Dec
Date Detail:
Created Date:  1995-03-28     Completed Date:  1995-03-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9102703     Medline TA:  J Heart Lung Transplant     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  998-1002     Citation Subset:  IM    
Affiliation:
Department of Surgery III, Nara Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Airway Resistance
Allopurinol / pharmacology
Animals
Blood Pressure
Dogs
Free Radicals
Hypertonic Solutions / pharmacology
Lipid Peroxidation*
Lung / metabolism*
Lung Transplantation*
Organ Preservation*
Pulmonary Artery / physiopathology
Superoxide Dismutase / pharmacology
Chemical
Reg. No./Substance:
0/Euro-Collins' solution; 0/Free Radicals; 0/Hypertonic Solutions; 315-30-0/Allopurinol; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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