Document Detail


Protection against infertility in a BALB/c mouse salpingitis model by intranasal immunization with the mouse pneumonitis biovar of Chlamydia trachomatis.
MedLine Citation:
PMID:  8039906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Female BALB/c mice were immunized intranasally with the mouse pneumonitis biovar of Chlamydia trachomatis and subsequently challenged in the ovarian bursa (C. trachomatis immunized, C. trachomatis challenged). Two groups of mice served as controls. One group was sham immunized intranasally with mock-infected HeLa 229 cell extracts and was challenged in the ovarian bursa with C. trachomatis MoPn (sham immunized, C. trachomatis challenged). The second control group was sham immunized and not challenged (sham immunized, nonchallenged). Before challenge, the C. trachomatis-immunized, C. trachomatis-challenged animals mounted a significant humoral response as shown by high immunoglobulin G (IgG), IgM, and IgA levels and high levels of neutralizing antibodies in serum and moderate IgG and IgA titers in vaginal secretions. Reactivity by Western blot (immunoblot) to the lipopolysaccharide, 30-, 40- (major outer membrane protein), and 60-kDa cysteine-rich proteins and 75- and 100-kDa chlamydial components could be demonstrated. However, reactivity to the 60-kDa heat shock protein was only observed 22 days after challenge. In addition, this group of animals mounted a significant immune response to chlamydial antigens, as shown by a lymphocyte proliferation assay, compared with the sham-immunized nonchallenged mice. After intrabursal challenge, there was no C. trachomatis shedding from the vagina in the C. trachomatis-immunized, C. trachomatis-challenged animals, while 63% of the sham-immunized, C. trachomatis-challenged mice had a positive C. trachomatis culture. In addition, histological sections from the genital tract showed, at 2 weeks postchallenge, a marked acute inflammatory reaction in the sham-immunized, C. trachomatis-challenged animals while in the C. trachomatis-immunized, C. trachomatis-challenged mice there was minimal inflammatory reaction. When the animals were mated, only 12% of the mice from the sham-immunized, C. trachomatis-challenged mice were fertile. In contrast, 94 and 80% of the sham-immunized, nonchallenged and C. trachomatis-immunized, C. trachomatis-challenged mice, respectively, were fertile.
Authors:
S Pal; T J Fielder; E M Peterson; L M de la Maza
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-08-24     Completed Date:  1994-08-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3354-62     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of California, Irvine 92717-4800.
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MeSH Terms
Descriptor/Qualifier:
Administration, Intranasal
Animals
Antibodies, Bacterial / analysis
B-Lymphocytes / immunology
Blotting, Western
Chlamydia Infections / complications*,  pathology
Chlamydia trachomatis / immunology*
Female
Infertility, Female / prevention & control*
Male
Mice
Mice, Inbred BALB C
Salpingitis / complications*,  pathology
Vaccination
Grant Support
ID/Acronym/Agency:
AI-26807/AI/NIAID NIH HHS; AI-30499/AI/NIAID NIH HHS; AI-32248/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial
Comments/Corrections

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