Document Detail

Protection against acute paraquat toxicity by ambroxol.
MedLine Citation:
PMID:  22358946     Owner:  NLM     Status:  In-Data-Review    
One of the earliest biochemical effects induced by the herbicide paraquat (PQ) is damage to type II pneumocytes with consequent depletion of surfactant (Skillrud and Martin, 1984). We made a series of studies on the possible protective effect of drug ambroxol, which induces surfactant synthesis from alveolar type II cells (Post et al. 1983). The cell line A-549, exposedin vitro to PQ concentrations ranging from 0.5×10(-4) to 2×10(-3) M, showed a significant dose-dependent loss of viability. Ambroxol (10 mg/ml) pretreated cells were more resistant to PQ, their viability starting to decrease from a PQ concentration of 0.8×10(-3) M. Membrane microviscosity was measured on the same cells. Cells treated with PQ alone showed a reduction of membrane microviscosity which was significantly counteracted by ambroxol pretreatment. The curves for membrane microviscosity of PQ and ambroxol-plus-PQ-treated cells overlapped those for cell viability, indicating that the stimulation of surfactant synthesisin vitro may be a prerequisite for counteracting some of the precocious effects of PQ. Partial protection from PQ- induced mortality was also obtainedin vivo.
L Perin; M Donnini; L Diomede; M Romano; M T Tacconi; M Luisetti; M Salmona
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  5     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1991 Feb 
Date Detail:
Created Date:  2012-02-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  25-7     Citation Subset:  -    
Laboratorio di Enzimologia, Istituto di Ricerche Farmacologiche "Mario Negri", Via Eritrea62, 20157, Milan, Italy.
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