Document Detail


Protection of exenatide for retinal ganglion cells with different glucose concentrations.
MedLine Citation:
PMID:  22727809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exendin-4 is a peptide resembling glucagon-like peptide-1 (GLP-1), which has protective effects on nerve cells. However, the effects of Exendin-4 on retinal ganglion cells (RGC) are still under clear. The purpose of the present study is to demonstrate that exenatide prevents high- or low-glucose-induced retinal ganglion cell impairment. We observed the expression of GLP-1R in RGC-5 cells by immunofluorescence and Western blot. To investigate the effect of exenatide on RGC-5 cells incubated different glucose concentrations, CCK-8 measured the survival rates and electron microscopy detected cellular injury. The expression levels of Bcl-2 and Bax were analyzed by immunocytochemistry and Western blot. Exenatide protects RGC-5 from high- or low-glucose-induced cellular injury and the optimum concentration was 0.5μg/ml. Exenatide can inhibit high- or low-glucose-induced mitochondrial changes. Exenatide protects RGC-5 from high- or low-glucose-induced Bax increased and Bcl-2 decreased. Furthermore, the protective effect of exenatide could be inhibited by Exendin (9-39). These findings indicate that exenatide shows a neuroprotective effect for different glucose concentrations-induced RGC-5 cells injury. Exenatide could protect RGC-5 cells from degeneration or death, which may protect retinal function and have a potential value for patients with diabetic retinopathy.
Authors:
Zheng Fu; Hong-Yu Kuang; Ming Hao; Xin-Yuan Gao; Yu Liu; Ning Shao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-19
Journal Detail:
Title:  Peptides     Volume:  37     ISSN:  1873-5169     ISO Abbreviation:  Peptides     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-24     Completed Date:  2013-01-07     Revised Date:  2013-03-19    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Endocrinology, Ningde Hospital, 352100 Fujian Province, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Survival / drug effects
Cytoprotection
Glucose / pharmacology,  physiology*
Mitochondria / drug effects,  metabolism,  ultrastructure
Organelle Shape / drug effects
Peptides / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Rats
Rats, Sprague-Dawley
Receptors, Glucagon / agonists,  metabolism
Retinal Ganglion Cells / drug effects*,  metabolism
Venoms / pharmacology*
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Bax protein, rat; 0/Peptides; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Glucagon; 0/Venoms; 0/bcl-2-Associated X Protein; 0/glucagon-like peptide-1 receptor; 141732-76-5/exenatide; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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