Document Detail

Protease activity in gingival crevicular fluid from discrete periodontal sites in humans with periodontitis or gingivitis.
MedLine Citation:
PMID:  2196865     Owner:  NLM     Status:  MEDLINE    
Sensitive fluorogenic assays were used to compare the protease activities of fluid collected from eight such sites in each of 21 adult patients with gingivitis and 22 with periodontitis. The degradation of N-carbobenzoxy-gly-gly-arginine-AMC, L-arginine-AMC, glyproline-AMC, L-leucine-AMC, N-alpha-benzoyl-L-arginine-AMC, N-[p-toluenesulphonyl]-gly-pro-arginine-AMC, N-tert-butoxycarbonyl-leu-ser-thr-arginine-AMC, N-tert-butoxycarbonyl-ileu-glut-gly-arginine-AMC and N-tert-butoxycarbonyl-val-leu-lysine-AMC was significantly greater by fluid from the periodontitis group. The specific rates of degradation of L-arginine-AMC, gly-proline-AMC, N-alpha-benzoyl-L-arginine-AMC and N-[p-toluene-sulphonyl]gly-pro-arginine-AMC were significantly greater in that group, indicating that the composition of their gingival crevicular fluid was different from that of the gingivitis group. Discriminant analysis of the substrate hydrolysis data alone correctly identified 77.6% of sites with sensitivity and specificity values of 73.3 and 82.1%, respectively. The predictive value of these assays requires further investigation, but it is possible that they will prove useful for monitoring the success of periodontal treatment.
D Beighton; J R Radford; M N Naylor
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  35     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1990  
Date Detail:
Created Date:  1990-08-23     Completed Date:  1990-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  329-35     Citation Subset:  D; IM    
Hunterian Dental Research Unit, London Hospital Medical College, Whitechapel, England.
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MeSH Terms
Chronic Disease
Coumarins / diagnostic use
Dental Plaque Index
Endopeptidases / metabolism*
Fluorescent Dyes / diagnostic use
Gingival Crevicular Fluid / metabolism*
Gingivitis / enzymology*,  metabolism*
Periodontal Index
Periodontitis / enzymology*
Periodontium / enzymology*
Predictive Value of Tests
Reg. No./Substance:
0/Coumarins; 0/Fluorescent Dyes; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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