Document Detail


Protamine inhibits coronary collateral development in a canine model of repetitive coronary occlusion.
MedLine Citation:
PMID:  7532374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protamine has been demonstrated to inhibit angiogenesis in vitro and in vivo; however, its effect on coronary collateral development has not been examined. The present investigation tested the hypothesis that subcutaneously administered protamine inhibits canine coronary collateral development in response to chronic myocardial ischemia. Dogs underwent daily, repetitive, 2-min, left anterior descending coronary artery (LAD) occlusions for 22 consecutive days. Regional myocardial blood flow (radioactive microspheres), LAD segment shortening, and coronary flow debt repayment were measured in saline-treated (n = 7) and protamine-treated (n = 6) dogs on days 1, 8, 15, and 22. Coronary collateral development in saline-treated dogs was demonstrated by time-dependent significant (P < 0.05) increases in collateral blood flow to ischemic myocardium [day 1 0.10 +/- 0.01 vs. day 22 0.88 +/- 0.05 (SE) ml.min-1.g-1], progressive normalization of myocardial contractile function during LAD occlusion, and successive reduction in flow debt repayment. In contrast, protamine treatment significantly attenuated, increases in collateral perfusion (day 1 0.13 +/- 0.02 vs. day 22 0.36 +/- 0.03 ml.min-1.g-1). Regional contractile dysfunction and postocclusive reactive hyperemic responses were sustained over time in protamine-treated compared with saline-treated dogs. The results demonstrate that protamine inhibits coronary collateral development in response to chronic myocardial ischemia.
Authors:
J R Kersten; P S Pagel; D C Warltier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  268     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-20     Completed Date:  1995-03-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H720-8     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226.
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MeSH Terms
Descriptor/Qualifier:
Animals
Collateral Circulation / drug effects*
Coronary Circulation / drug effects*
Coronary Disease / physiopathology*
Dogs
Hemodynamics / drug effects
Hyperemia / physiopathology
Myocardial Contraction / drug effects
Neovascularization, Pathologic / prevention & control*
Protamines / pharmacology*
Recurrence
Grant Support
ID/Acronym/Agency:
GM-08377/GM/NIGMS NIH HHS; HL-36144/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Protamines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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