Document Detail


Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3. Requirement for protein synthesis.
MedLine Citation:
PMID:  557039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stimulation of prostaglandin synthesis in transformed mouse fibroblasts by serum, thrombin, and bradykinin was blocked by actinomycin D and cycloheximide. These RNA and protein synthesis inhibitors did not affect prostaglandin synthetase in vitro or in vivo; nor did they affect the acylation of arachidonic acid into phospholipids. Serum-stimulated release of arachidonic acid and prostaglandins from [3H]arachidonic acid-labeled cells also was inhibited by actinomycin D and cycloheximide. RNA and protein synthesis appear to be required for expression of phospholipase activity; a prerequisite for prostaglandin synthesis by these cells.
Authors:
S S Pong; S L Hong; L Levine
Related Documents :
3685399 - The effects of danazol, mefenamic acid, norethisterone and a progesterone-impregnated c...
4215079 - Prostaglandin endoperoxides. novel transformations of arachidonic acid in human platelets.
2609239 - Metabolic effects of prostaglandin e1 on human skeletal muscle with special regard to t...
1007749 - Essential fatty acids and prostaglandins.
7827039 - Bromoacetamido analogs of indomethacin and mefenamic acid as affinity-labeling agents a...
8629459 - Concentrations of various arachidonic acid metabolites in menstrual fluid are associate...
18558769 - Synthesis of alpha-trifluoromethyl-beta-lactams and esters of beta-amino acids via 1,3-...
8274289 - Effects of sphingosine, isoquinoline and tannic acid on the human tape-stripping model ...
18174199 - Role of beta-lactamase inhibitors in enterobacterial isolates producing extended-spectr...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  252     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-04-15     Completed Date:  1977-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1408-13     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology
Arachidonic Acids / metabolism
Cell Line
Cell Transformation, Neoplastic
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Emetine / pharmacology
Kinetics
Methylcholanthrene / pharmacology*
Mice
Prostaglandins / biosynthesis*
Prostaglandins E / biosynthesis
Prostaglandins F / biosynthesis
Protein Biosynthesis / drug effects*
Tosyl Compounds / pharmacology
Transcription, Genetic / drug effects
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Arachidonic Acids; 0/Prostaglandins; 0/Prostaglandins E; 0/Prostaglandins F; 0/Tosyl Compounds; 483-18-1/Emetine; 50-76-0/Dactinomycin; 56-49-5/Methylcholanthrene; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mechanism of action of uridine diphoglucose dehydrogenase. Evidence for an essential lysine residue ...
Next Document:  Studies on mitochondrial structure and function in Physarum polycephalum. V. Behaviour of mitochondr...