Document Detail


Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets.
MedLine Citation:
PMID:  4215079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arachidonic acid incubated with human platelets was converted into three compounds, 12L-hydroxy-5,8,10,14-eicosatetraenoic acid, 12L-hydroxy-5,8,10-heptadecatrienoic acid, and the hemiacetal derivative of 8-(1-hydroxy-3-oxopropyl)-9,12L-dihydroxy-5,10-heptadecadienoic acid. The formation of the two latter compounds from arachidonic acid proceeded by pathways involving the enzyme, fatty acid cyclo-oxygenase, in the initial step and with the prostaglandin endoperoxide, PGG(2), as an intermediate. The first mentioned compound was formed from 12L-hydroperoxy-5,8,10,14-eicosatetraenoic acid, which in turn was formed from arachidonic acid by the action of a novel lipoxygenase. Aspirin and indomethacin inhibited the fatty acid cyclo-oxygenase but not the lipoxygenase, whereas 5,8,11,14-eicosatetraynoic acid inhibited both enzymes. The almost exclusive transformation of the endoperoxide structure into non-prostaglandin derivatives supports the hypothesis that the endoperoxides can participate directly and not by way of the classical prostaglandins in regulation of cell functions. The observed transformations of arachidonic acid in platelets also explain the aggregating effect of this acid.
Authors:
M Hamberg; B Samuelsson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 Sep 
Date Detail:
Created Date:  1975-01-25     Completed Date:  1975-01-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3400-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acids / metabolism*
Aspirin / pharmacology
Blood Platelets / metabolism*
Borohydrides / metabolism
Carbon Radioisotopes
Chromatography, Thin Layer
Fatty Acids, Unsaturated / pharmacology
Humans
Indomethacin / pharmacology
Lipoxygenase / metabolism
Mass Spectrometry
Oxygenases / antagonists & inhibitors,  metabolism
Peroxides / metabolism
Platelet Aggregation
Prostaglandins / biosynthesis*
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Borohydrides; 0/Carbon Radioisotopes; 0/Fatty Acids, Unsaturated; 0/Peroxides; 0/Prostaglandins; 50-78-2/Aspirin; 53-86-1/Indomethacin; EC 1.13.-/Oxygenases; EC 1.13.11.12/Lipoxygenase
Comments/Corrections

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