| Prostaglandin H synthase Type 2 is differentially expressed in endometrium based on pregnancy status in pony mares and responds to oxytocin and conceptus secretions in explant culture. | |
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MedLine Citation:
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PMID: 19443143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The equine embryo must signal its presence to the uterus for pregnancy to continue to term. Mobility of the conceptus throughout the uterus is crucial for its survival, and this action presumably permits the conceptus to transmit its antiluteolytic signal to the endometrium. Studies were completed to establish whether this unidentified antiluteolytic signal targets prostaglandin G/H synthase 2 (PGHS2), a rate limiting enzyme in converting arachidonic acid to prostaglandins (PGs). In the first study, quantitative RT-PCR was used to determine the relative abundance of PGHS2 mRNA in endometrium derived from estrous cyclic and pregnant mares on day 14 post-ovulation. PGHS2 mRNA abundance was substantially greater in endometrium from estrous cyclic mares. Additional studies were completed to better understand PGHS2 in equine endometrium. An estrogen and progesterone treatment regimen in ovariectomized mares was developed as a test model for detecting endometrial PGHS2 mRNA. Also, exposing endometrial explants to conceptus secretions (conditioned culture medium) decreased PGHS2 mRNA abundance whereas exposing explants to oxytocin increased PGHS2 mRNA abundance. Exposure to conceptus secretions also decreased PGF2alpha concentrations in explant-conditioned medium whereas oxytocin supplementation increased PGF2alpha concentrations in medium. These data support the hypothesis that PGHS2 is a target for the antiluteolytic signal produced by equine conceptuses during early pregnancy. Also, the endometrial explant culture system used for these studies can serve as a model for identifying and characterizing the maternal recognition of pregnancy factor in equids. |
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Authors:
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Alan D Ealy; Michelle L Eroh; Daniel C Sharp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-05 |
Journal Detail:
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Title: Animal reproduction science Volume: 117 ISSN: 1873-2232 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-11-10 Completed Date: 2010-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 99-105 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Culture Media, Conditioned Dinoprost / analysis Embryo, Mammalian / secretion Endometrium / enzymology* Female Gene Expression / drug effects* Gestational Age Horses / metabolism* Oxytocin / pharmacology* Pregnancy Prostaglandin-Endoperoxide Synthases / genetics* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Tissue Culture Techniques / veterinary |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/RNA, Messenger; 50-56-6/Oxytocin; 551-11-1/Dinoprost; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
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