Document Detail

Prostaglandin E2 inhibits in vitro and in vivo lymphocyte responses in allogeneic transplantation.
MedLine Citation:
PMID:  2142409     Owner:  NLM     Status:  MEDLINE    
Prostaglandin E2 (PGE2) has been shown to a clear role in the suppression of immune responses after burn and trauma injury. This probably results from inhibition of interleukin-2 production. This study examined the effects of PGE2 in vivo on the survival of solid-organ allografts and in vitro on the rat allogeneic mixed lymphocyte response. Administration of 16,16-dimethyl prostaglandin E2 (DMPGE2), a stable analogue of PGE2, significantly prolonged the survival of heterotopic cardiac allografts from ACI to LBN rats: 10.4 +/- 1.7 days versus 5.7 +/- 1.1 days (mean +/- standard error of the mean) (p less than or equal to 0.001). In 1 animal, DMPGE2 apparently led to the induction of long-term tolerance. Mixed lymphocyte cultures using splenocytes from naive LBN and ACI rats to which DMPGE2 was added showed a dose-dependent suppression of the mixed lymphocyte response with concentrations as low as 1 x 10(-7) mol/L. Splenocytes harvested from treated animals with functioning but histologically rejecting hearts demonstrated a marked decrease in mixed lymphocyte response to donor (ACI) stimulators compared with naive LBN controls (3,804 +/- 603 versus 27,395 +/- 2,668 cpm, n = 4), but maintained a normal response to third-party (Wistar Furth) stimulators. We conclude that DMPGE2 suppressed solid-organ allograft rejection, inhibited the allogeneic mixed lymphocyte response, and induced donor-specific in vitro hyporesponsiveness.
C D Stone; B R Rosengard; S M Boorstein; R C Robbins; H A Hennein; R E Clark
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  49     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-13     Completed Date:  1990-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  927-30; discussion 931     Citation Subset:  AIM; IM    
Surgery Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
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MeSH Terms
16,16-Dimethylprostaglandin E2 / administration & dosage,  pharmacology*
Dose-Response Relationship, Drug
Graft Survival / drug effects*
Heart Transplantation / immunology*
Immunosuppressive Agents*
Lymphocyte Activation / drug effects*
Lymphocyte Culture Test, Mixed
Lymphocytes / drug effects,  immunology
Prostaglandins E, Synthetic / pharmacology*
Rats, Inbred ACI
Rats, Inbred Lew
Rats, Inbred Strains
Rats, Inbred WF
Spleen / cytology
Time Factors
Reg. No./Substance:
0/Immunosuppressive Agents; 0/Prostaglandins E, Synthetic; 39746-25-3/16,16-Dimethylprostaglandin E2

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