Document Detail


Prostaglandin E1 transported into cells blocks the apoptotic signals induced by nerve growth factor deprivation.
MedLine Citation:
PMID:  10217267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuronal apoptosis in rat pheochromocytoma PC12 cells, which was confirmed by TUNEL (terminal transferase-mediated dUTP-biotin nick end-labeling) staining and detection of chromatin condensation, appeared within 8 h after nerve growth factor (NGF) deprivation. Prostaglandin (PG) E1 (10(-7)-10(6) M) reduced the incidence of apoptotic cell death in PC12 cells. The genes encoding PG transporter specific to prostaglandins such as PGE2 or PGF2alpha were expressed in the cell lines as shown by RT-PCR. Bromcresol green, an inhibitor of PG transporter, reversed the antiapoptotic effect of PGE1. Moreover, treatment of PC12 cells with an antisense oligonucleotide corresponding to PG transporter cDNA also blocked the inhibitory effects of PGE1 on apoptotic cell death. In addition, PGE1 counteracted the increased activities of stress-activated protein kinase/cJun N-terminal kinase within 1-2 h after NGF deprivation in PC12 cells. These results indicated that the antiapoptotic effect of PGE1 in NGF-deprived PC12 cells was achieved by inhibitory signals following uptake into neurons through the PG transporter.
Authors:
T Kawamura; S Horie; T Maruyama; T Akira; T Imagawa; N Nakamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  72     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1907-14     Citation Subset:  IM    
Affiliation:
Osaka Laboratories, Pharmaceutical Research Division, Yoshitomi Pharmaceutical Industries, Ltd., Japan.
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MeSH Terms
Descriptor/Qualifier:
Alprostadil / pharmacokinetics,  pharmacology*
Animals
Antiporters / metabolism,  physiology
Apoptosis / drug effects*,  physiology
Biological Transport / physiology
Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
Cell Differentiation / physiology
Cyclic AMP / metabolism
DNA-Binding Proteins / metabolism,  physiology
Intracellular Membranes / physiology
Nerve Growth Factors / deficiency*,  pharmacology
Neurons / drug effects*,  metabolism,  physiology
Organic Anion Transporters
PC12 Cells / drug effects,  metabolism,  pathology
Rats
Signal Transduction / drug effects*,  physiology
Chemical
Reg. No./Substance:
0/Antiporters; 0/DNA-Binding Proteins; 0/Nerve Growth Factors; 0/Organic Anion Transporters; 0/Slco2a1 protein, rat; 60-92-4/Cyclic AMP; 745-65-3/Alprostadil; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases

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