| Prostaglandin E1 receptor from mouse mastocytoma P-815 cells couples to 60 kDa GTP-binding protein. | |
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MedLine Citation:
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PMID: 1657195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The stable [3H]prostaglandin E1 (PGE1)-bound receptor, which couples to 60 kDa GTP-binding protein, from membranes of mouse mastocytoma P-815 cells has been purified and characterized. When the membranes were preincubated with [3H]PGE1 for 60 min at 37 degrees C, the dissociation of the ligand from the receptor was remarkably decreased, even in the presence of GTP gamma S. The stable [3H]PGE1-bound receptor complex was solubilized with 6% digitonin. The solubilized [3H]PGE1 receptor was eluted with [35S]GTP gamma S bindings activity from an Ultrogel AcA44 column. The fractions containing activities of both [3H]PGE1 and [35S]GTP gamma S bindings were further purified by column chromatographies on wheat germ agglutinin (WGA)-agarose and phenyl-Sepharose CL-4B. The partially purified [3H]PGE1-bound receptor was affinity-labeled with [14C]5'-p-fluorosulfonylbenzoylguanosine and a protein with a molecular mass of 60 kDa was detected. These results suggest that the ligand-bound PGE1 receptor of P-815 cells associates with a novel GTP-binding protein with a molecular mass of 60 kDa. |
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Authors:
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H Hashimoto; R Ogino; H Saeki; K Yatsunami; A Ichikawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1095 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-12-11 Completed Date: 1991-12-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 140-4 Citation Subset: IM |
Affiliation:
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Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism Animals Chromatography GTP-Binding Proteins / metabolism* Guanosine 5'-O-(3-Thiotriphosphate) / metabolism Guanosine Triphosphate / metabolism Kinetics Mice Prostaglandins E / metabolism* Receptors, Prostaglandin / isolation & purification, metabolism* Receptors, Prostaglandin E Signal Transduction Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Prostaglandins E; 0/Receptors, Prostaglandin; 0/Receptors, Prostaglandin E; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 86-01-1/Guanosine Triphosphate; EC 3.6.1.-/GTP-Binding Proteins; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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