Document Detail


Prospects of riboflavin carrier protein (RCP) as an antifertility vaccine in male and female mammals.
MedLine Citation:
PMID:  9459278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Riboflavin carrier protein (RCP) is obligatorily involved in yolk deposition of the vitamin, riboflavin, in the developing oocyte of the hen. The production of this protein is inducible by oestrogen. It is evolutionarily conserved in terms of its physicochemical, immunological and functional characteristics. It is the prime mediator of vitamin supply to the developing fetus in mammals, including primates. Passive immunoneutralization of the protein terminates pregnancy in rats. Active immunization of rats and bonnet monkeys with avian RCP prevents pregnancy without causing any adverse physiological effects of the mother in terms of her vitamin status, reproductive cycles or reproductive-endocrine profile. Denatured, linearized RCP is more effective in eliciting neutralizing antibodies capable of interfering with embryonic viability either before or during peri-implantation stages. Two defined stretches of sequential epitopes, one located at the N-terminus and the other at the C-terminus of the protein have been identified. Active immunization with either of these epitopes conjugated with diphtheria toxoid curtails pregnancy in rats and monkeys. Immunohistochemical localization of RCP on ovulated oocytes and early embryos shows that the antibodies cause degeneration only of early embryos. RCP is produced intra-testicularly and becomes localized on acrosomal surface of mammalian spermatozoa. Active immunization of male rats and monkeys with denatured RCP markedly reduces fertility by impairing the fertilizing potential of spermatozoa. These findings suggest that RCP, or its defined fragments, could be a novel, first generation vaccine for regulating fertility in both the sexes.
Authors:
P R Adiga; S Subramanian; J Rao; M Kumar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Human reproduction update     Volume:  3     ISSN:  1355-4786     ISO Abbreviation:  Hum. Reprod. Update     Publication Date:    1997 Jul-Aug
Date Detail:
Created Date:  1998-03-04     Completed Date:  1998-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9507614     Medline TA:  Hum Reprod Update     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  325-34     Citation Subset:  IM    
Affiliation:
Centre for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore. adiga@serc.iisc.ernet.in
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Carrier Proteins / analysis,  chemistry,  immunology*
Chickens
Contraception, Immunologic / methods*
Female
Humans
Male
Mammals
Membrane Transport Proteins*
Molecular Sequence Data
Pregnancy
Riboflavin / metabolism
Vaccines* / immunology
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Transport Proteins; 0/Vaccines; 0/riboflavin-binding protein; 83-88-5/Riboflavin
Comments/Corrections
Comment In:
Hum Reprod Update. 1997 Jul-Aug;3(4):299-300   [PMID:  9459275 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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