Document Detail


Prospective study of sensitization and food allergy to flaxseed in 1317 subjects.
MedLine Citation:
PMID:  20648772     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Foods containing flaxseed proteins rich inpolyunsaturatedfatty acids are new on the market. OBJECTIVES: In a population of patients attending the allergology department, we evaluated the frequency of sensitization to flaxseed, characterized allergens and looked for modifications related to industrial processing. METHODS: Natural, heated and extruded flaxseeds were tested using prick-in-prick tests (PIP using the fresh seed), SDS PAGE, immunoblots, immunoblot inhibition and Fourier Transform Infrared (FTIR) spectroscopy. RESULTS: PIP tests to natural flaxseed were positive in 5.8% of the 1317 patients. 73 of 77 PIP-positive patients were atopic. There was cross-reactivity with five seeds. peanut, soybean, rapeseed, lupine and wheat, and with rape pollen. Immunoblot inhibition by bromelain confirmed the presence of specific IgE to cross-reactive carbohydrate determinants (CCD). 0.15% of this population presented with food allergy to flaxseed and positive PIP to heated and extruded flaxseed. Two sera showed that clinically relevant allergens in industrial products had MW between 25 and 38 kDa. Sensitization to processed flaxseed characterized only the allergic subjects. FTIR spectroscopy showed major modifications in 3 and alpha structures following industrial processing. CONCLUSION: Positive prick tests to natural flaxseed were mainly due to cross-reactions. Flaxseed allergy is rare and could be detected by PIP to heated extruded flaxseed. Increasing consumption callsfor monitoring of clinical risk.
Authors:
S Fremont; D A Moneret-Vautrin; P Franck; M Morisset; A Croizier; F Codreanu; G Kanny
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European annals of allergy and clinical immunology     Volume:  42     ISSN:  1764-1489     ISO Abbreviation:  Eur Ann Allergy Clin Immunol     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-22     Completed Date:  2010-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466614     Medline TA:  Eur Ann Allergy Clin Immunol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  103-11     Citation Subset:  IM    
Affiliation:
Laboratoire de Biochimie et Biologie Moléculaire, Hôpital Central, Nancy.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Blotting, Western
Carbohydrates / immunology
Child
Child, Preschool
Cross Reactions
Female
Flax / immunology*
Food Hypersensitivity / diagnosis*
Humans
Immunoglobulin E / blood
Infant
Male
Middle Aged
Prospective Studies
Skin Tests
Spectroscopy, Fourier Transform Infrared
Chemical
Reg. No./Substance:
0/Carbohydrates; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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