Document Detail

Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.
MedLine Citation:
PMID:  20171613     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy.
DESIGN: Prospective randomized.
SETTING: Academically affiliated, private fertility center.
PATIENT(S): Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were randomized to slow-rate freezing or vitrification of supernumerary (more than nine) oocytes.
INTERVENTION(S): Oocytes were frozen or vitrified, and upon request oocytes were thawed or warmed, respectively.
MAIN OUTCOME MEASURE(S): Oocyte survival, fertilization, embryo development, and clinical pregnancy.
RESULT(S): Patient use has resulted in 30 thaws and 48 warmings. Women's age at time of cryopreservation was similar. Oocyte survival was significantly higher following vitrification/warming (81%) compared with freezing/thawing (67%). Fertilization was more successful in oocytes vitrified/warmed compared with frozen/thawed. Fertilized oocytes from vitrification/warming had significantly better cleavage rates (84%) compared with freezing/thawing (71%) and resulted in embryos with significantly better morphology. Although similar numbers of embryos were transferred, embryos resulting from vitrified oocytes had significantly enhanced clinical (38%) pregnancy rates compared with embryos resulting from frozen oocyte (13%). Miscarriage and/or spontaneous abortion rates were similar.
CONCLUSION(S): Our results suggest that vitrification/warming is currently the most efficient means of oocyte cryopreservation in relation to subsequent success in establishing pregnancy.
Gary D Smith; Paulo C Serafini; Joyce Fioravanti; Isaac Yadid; Marcio Coslovsky; Pericles Hassun; José Roberto Alegretti; Eduardo L Motta
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Publication Detail:
Type:  Comparative Study; Journal Article; Randomized Controlled Trial     Date:  2010-02-19
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-27     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2088-95     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA.
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MeSH Terms
Cryopreservation / methods*
Embryo Transfer / methods
Fertilization / physiology
Ovulation Induction / methods
Pregnancy Rate
Sperm Injections, Intracytoplasmic / methods
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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