| Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification. | |
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MedLine Citation:
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PMID: 20171613 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN: Prospective randomized. SETTING: Academically affiliated, private fertility center. PATIENT(S): Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were randomized to slow-rate freezing or vitrification of supernumerary (more than nine) oocytes. INTERVENTION(S): Oocytes were frozen or vitrified, and upon request oocytes were thawed or warmed, respectively. MAIN OUTCOME MEASURE(S): Oocyte survival, fertilization, embryo development, and clinical pregnancy. RESULT(S): Patient use has resulted in 30 thaws and 48 warmings. Women's age at time of cryopreservation was similar. Oocyte survival was significantly higher following vitrification/warming (81%) compared with freezing/thawing (67%). Fertilization was more successful in oocytes vitrified/warmed compared with frozen/thawed. Fertilized oocytes from vitrification/warming had significantly better cleavage rates (84%) compared with freezing/thawing (71%) and resulted in embryos with significantly better morphology. Although similar numbers of embryos were transferred, embryos resulting from vitrified oocytes had significantly enhanced clinical (38%) pregnancy rates compared with embryos resulting from frozen oocyte (13%). Miscarriage and/or spontaneous abortion rates were similar. CONCLUSION(S): Our results suggest that vitrification/warming is currently the most efficient means of oocyte cryopreservation in relation to subsequent success in establishing pregnancy. |
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Authors:
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Gary D Smith; Paulo C Serafini; Joyce Fioravanti; Isaac Yadid; Marcio Coslovsky; Pericles Hassun; José Roberto Alegretti; Eduardo L Motta |
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Publication Detail:
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Type: Comparative Study; Journal Article; Randomized Controlled Trial Date: 2010-02-19 |
Journal Detail:
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Title: Fertility and sterility Volume: 94 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-27 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 2088-95 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cryopreservation / methods* Embryo Transfer / methods Female Fertilization / physiology Freezing Humans Oocytes* Ovulation Induction / methods Pregnancy Pregnancy Rate Sperm Injections, Intracytoplasmic / methods Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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