Document Detail

Prospective evaluation of pyrosequencing for the rapid detection of isoniazid and rifampin resistance in clinical Mycobacterium tuberculosis isolates.
MedLine Citation:
PMID:  18719956     Owner:  NLM     Status:  MEDLINE    
A pyrosequencing-based method for the rapid detection of isoniazid (INH) and rifampin (RIF) resistance in Mycobacterium tuberculosis was evaluated in clinical practice. The method can detect the INH resistance-causing katG315 mutation, and all mutations in the RIF resistance-determining rpoB core region, in less than 6 h from cultured isolates. The method was first validated with 42 isolates, and was subsequently prospectively evaluated with 91 isolates, including clinical isolates and external quality control assessment strains, over a period of 2.5 years. The pyrosequencing results of clinical isolates were available, on average, 19 days earlier (median 19 days; range 3-43 days) than conventional susceptibility testing results. The composite data showed that the sensitivity of pyrosequencing for detecting resistance correctly was 66.7% for INH and 97.4% for RIF. The specificity of pyrosequencing was 100% for both drugs. Acceptable sensitivity for detecting resistance and the rapidness of pyrosequencing make it a valuable tool in the clinical setting.
H J Marttila; J Mäkinen; M Marjamäki; H Soini
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-08-22
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  28     ISSN:  1435-4373     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-02-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  33-8     Citation Subset:  IM    
Mycobacterial Reference Laboratory, National Public Health Institute, Turku, Finland.
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MeSH Terms
Amino Acid Substitution / genetics
Antitubercular Agents / pharmacology*
Bacterial Proteins / genetics
Catalase / genetics
DNA, Bacterial / chemistry,  genetics*
Drug Resistance, Bacterial*
Isoniazid / pharmacology*
Microbial Sensitivity Tests / methods
Mutation, Missense
Mycobacterium tuberculosis / drug effects*,  genetics
Prospective Studies
Rifampin / pharmacology*
Sensitivity and Specificity
Sequence Analysis, DNA / methods*
Reg. No./Substance:
0/Antitubercular Agents; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/rpoB protein, Mycobacterium tuberculosis; 13292-46-1/Rifampin; 54-85-3/Isoniazid; EC; EC protein, Mycobacterium tuberculosis

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