Document Detail


Prospective BCR-ABL analysis by polymerase chain reaction (RT-PCR) in adult acute B-lineage lymphoblastic leukemia: reliability of RT-nested-PCR and comparison to cytogenetic data.
MedLine Citation:
PMID:  11753602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reliability of routine BCR-ABL RT-nested-PCR was evaluated in 1453 B-lineage ALL or hybrid leukemia at initial diagnosis by RT-nested-PCR. All BCR-ABL-positive (n = 642) and 176 BCR-ABL-negative samples underwent a second RT-PCR. In 518 patients, karyotyping and/or FISH was compared to the BCR-ABL status. The second RT-PCR revealed in 155/642 initially positive samples a divergent result (153 BCR-ABL-negative, two other transcripts) that in most cases turned out to be caused by contaminations in the first RT-nested-PCR. Confirmatory RT-PCR detected 2/176 false negative first RT-nested-PCR results. Thirty-nine specimens remained ambiguous despite different RT-PCR approaches. As far as cytogenetic evaluation and FISH is available (n = 23), the majority but not all patients with an ambiguous RT-PCR result were Ph-negative (n = 18). RT-nested-PCR and cytogenetics yielded in 346 of 383 evaluable samples a concordant result. Differing results are given and account in part to the lower sensitivity of karyotyping. Taken together, confirmed RT-PCR detected BCR-ABL fusion transcripts consistently in 487 out of 1453 ALL samples (c-ALL: 43%, pre-B ALL: 34%, pro-B ALL: 5%, B-ALL: 0%, hybrid leukemia: 5/11). Since false positive initial RT-nested-PCR data were frequent, either confirmatory second RT-PCR or FISH analysis is warranted to guarantee sensitive and reliable results of utmost clinical relevance.
Authors:
B Gleissner; H Rieder; E Thiel; C Fonatsch; L A Janssen; B Heinze; J W Janssen; C Schoch; N Goekbuget; J Maurer; D Hoelzer; C R Bartram
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia     Volume:  15     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-01-22     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  England    
Other Details:
Languages:  eng     Pagination:  1834-40     Citation Subset:  IM    
Affiliation:
Department of Hematology, University Hospital Benjamin Franklin, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Burkitt Lymphoma / diagnosis*,  genetics
Cytogenetic Analysis / standards
Diagnostic Errors
Fusion Proteins, bcr-abl / analysis,  genetics*
Humans
Philadelphia Chromosome
Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis*,  genetics
Prospective Studies
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction / standards*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Fusion Proteins, bcr-abl

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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