Document Detail

Pros and cons of immunochemical and enzymatic method in the diagnosis of Wilson's disease.
MedLine Citation:
PMID:  16940686     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Immunochemical method of measuring Ceruloplasmin (Cp) levels for the diagnosis of Wilson's disease has replaced enzymatic method for the main reason of being more sensitive and quantitative. SETTINGS AND DESIGN: In this study, we compared both the methods for various factors such as sensitivity, specificity and the time consumed in the diagnosis of Wilson's disease. MATERIALS AND METHODS: Serum samples from patients (n=33) with a provisional diagnosis of Wilson's disease were analyzed for Cp levels by enzymatic copper oxidase method and immunochemical method using polyclonal antibodies specific to Cp embedded in agar. STATISTICAL ANALYSIS: Pearson's regression analysis was performed to compare the two methods. RESULTS: The mean Cp obtained by immunochemical method is 5.87 mm +/- 1.17 and by enzymatic method, it is 0.37 (Optical Density) +/- 0.136. Pearson's Regression analysis of the measurements showed a good correlation with an 'r' value of 0.765 between the two methods. CONCLUSION: A good correlation indicated that these two tests are comparable and thus both these methods can be used together for a definitive and better diagnosis of Wilson's disease.
J V Gnanou; V G Thykadavil; V Thuppil
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Indian journal of medical sciences     Volume:  60     ISSN:  0019-5359     ISO Abbreviation:  Indian J Med Sci     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-30     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373023     Medline TA:  Indian J Med Sci     Country:  India    
Other Details:
Languages:  eng     Pagination:  371-5     Citation Subset:  IM    
University of Seychelles American Institute of Medicine, MAHE, Seychelles.
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MeSH Terms
Biological Assay
Ceruloplasmin / analysis*,  metabolism
Feasibility Studies
Hepatolenticular Degeneration / diagnosis*,  enzymology,  immunology
Oxidoreductases / metabolism*
Prospective Studies
Sensitivity and Specificity
Reg. No./Substance:
EC 1.-/Oxidoreductases; EC 1.16.-/copper oxidase; EC

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