| Propranolol preserves ultrastructure in adult cardiocytes exposed to anoxia/reoxygenation: a morphometric analysis. | |
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MedLine Citation:
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PMID: 1937138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protective effect of d,l-propranolol was studied using freshly isolated canine ventricular cardiocytes (1.5 x 10(6)/mL) exposed to 30 min anoxia (95% N2/5% CO2) and 0, 3, 20, and 45 min of reoxygenation (95% O2/5% CO2). In addition to preventing lipid peroxide formation, propranolol maintained cellular viability, and minimized ultrastructural alterations. In the absence of propranolol, the outer mitochondria become swollen and rounded up within the first few minutes of reoxygenation. The perinuclear mitochondrial area increased only slightly. We observed that the cellular injury process proceeded differentially from the exterior to the interior, with a mitochondrial area increase and outer membrane rupture. Sarcolemmal damage was also observed with prevalent blebbing and membrane loss. The Z-lines became wider and more diffuse with reoxygenation. Injury to the nuclear double membrane was observed. Incubation with propranolol showed significant protection during postanoxia reoxygenation. In contrast, the more water soluble beta-blocker atenolol only exhibited slight protection. In addition, d-propranolol (the non beta-blocking isomer) and the antioxidant enzymes, SOD and catalase, showed significant protection. These data support previous findings concerning the antioxidant properties of propranolol which appear to be independent of beta-receptor blockade. |
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Authors:
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A M Freedman; J H Kramer; I T Mak; M M Cassidy; W B Weglicki |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Free radical biology & medicine Volume: 11 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 1991 |
Date Detail:
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Created Date: 1991-12-09 Completed Date: 1991-12-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 197-206 Citation Subset: IM |
Affiliation:
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Department of Medicine, George Washington University Medical Center, Washington, DC 20037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / metabolism*, pathology Antioxidants Cell Survival / drug effects Dogs Heart Ventricles / cytology, drug effects, ultrastructure* Microscopy, Electron, Scanning Myocardial Reperfusion Injury / metabolism, pathology, prevention & control* Oxygen / pharmacology* Propranolol* |
| Grant Support | |
ID/Acronym/Agency:
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P01-HL38079/HL/NHLBI NIH HHS; R01-HL36418/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 525-66-6/Propranolol; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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