Document Detail

A proposal: Source of single strand DNA that elicits the SOS response.
MedLine Citation:
PMID:  23276924     Owner:  NLM     Status:  MEDLINE    
Chromosome replication is performed by numerous proteins that function together as a "replisome". The replisome machinery duplicates both strands of the parental DNA simultaneously. Upon DNA damage to the cell, replisome action produces single-strand DNA to which RecA binds, enabling its activity in cleaving the LexA repressor and thus inducing the SOS response. How single-strand DNA is produced by a replisome acting on damaged DNA is not clear. For many years it has been assumed the single-strand DNA is generated by the replicative helicase, which continues unwinding DNA even after DNA polymerase stalls at a template lesion. Recent studies indicate another source of the single-strand DNA, resulting from an inherently dynamic replisome that may hop over template lesions on both leading and lagging strands, thereby leaving single-strand gaps in the wake of the replication fork. These single-strand gaps are proposed to be the origin of the single-strand DNA that triggers the SOS response after DNA damage.
Chiara Indiani; Mike O'Donnell
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Landmark edition)     Volume:  18     ISSN:  1093-4715     ISO Abbreviation:  Front Biosci (Landmark Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-06-12     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  101612996     Medline TA:  Front Biosci (Landmark Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  312-23     Citation Subset:  IM    
Manhattan College 4513 Manhattan College Pkwy, Riverdale, NY 10471, USA.
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MeSH Terms
DNA Damage
DNA Helicases / metabolism
DNA Polymerase III / metabolism
DNA Replication
DNA, Single-Stranded / biosynthesis*
DnaB Helicases / metabolism
Models, Biological
SOS Response (Genetics) / physiology*
Grant Support
Reg. No./Substance:
0/DNA, Single-Stranded; EC 2.7.7.-/DNA Polymerase III; EC 3.1.-/DnaB Helicases; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/dnaB protein, E coli

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