Document Detail


A proposal: Source of single strand DNA that elicits the SOS response.
MedLine Citation:
PMID:  23276924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome replication is performed by numerous proteins that function together as a "replisome". The replisome machinery duplicates both strands of the parental DNA simultaneously. Upon DNA damage to the cell, replisome action produces single-strand DNA to which RecA binds, enabling its activity in cleaving the LexA repressor and thus inducing the SOS response. How single-strand DNA is produced by a replisome acting on damaged DNA is not clear. For many years it has been assumed the single-strand DNA is generated by the replicative helicase, which continues unwinding DNA even after DNA polymerase stalls at a template lesion. Recent studies indicate another source of the single-strand DNA, resulting from an inherently dynamic replisome that may hop over template lesions on both leading and lagging strands, thereby leaving single-strand gaps in the wake of the replication fork. These single-strand gaps are proposed to be the origin of the single-strand DNA that triggers the SOS response after DNA damage.
Authors:
Chiara Indiani; Mike O'Donnell
Related Documents :
18473724 - Helicases as prospective targets for anti-cancer therapy.
24924514 - Controlled hierarchical assembly of spider silk-dna chimeras into ribbons and raft-like...
10819984 - Strand-separating activity of hepatitis c virus helicase in the absence of atp.
23230454 - Nucleic acid amplification based diagnostic of lyme (neuro-)borreliosis - lost in the j...
1740384 - Isolation and characterization of a portal protein-dna complex from dsdna bacteriophage.
3314294 - Repair of uv-damaged dna in mammalian skin followed by the immunohistochemical method.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Landmark edition)     Volume:  18     ISSN:  1093-4715     ISO Abbreviation:  Front Biosci (Landmark Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-06-12     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  101612996     Medline TA:  Front Biosci (Landmark Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  312-23     Citation Subset:  IM    
Affiliation:
Manhattan College 4513 Manhattan College Pkwy, Riverdale, NY 10471, USA. chiara.indiani@manhattan.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
DNA Damage
DNA Helicases / metabolism
DNA Polymerase III / metabolism
DNA Replication
DNA, Single-Stranded / biosynthesis*
DnaB Helicases / metabolism
Models, Biological
SOS Response (Genetics) / physiology*
Grant Support
ID/Acronym/Agency:
GM38839/GM/NIGMS NIH HHS; P41 RR000862/RR/NCRR NIH HHS; R01 GM038839/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; EC 2.7.7.-/DNA Polymerase III; EC 3.1.-/DnaB Helicases; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/dnaB protein, E coli
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Non-syndromic thoracic aortic aneurysms and dissections--a genetic review.
Next Document:  BlyS: a potential hallmark of multiple myeloma.