| Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo. | |
| | |
MedLine Citation:
|
PMID: 18996224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Propofol, a rapidly acting, short duration, intravenous hypnotic anesthetic induction agent, is often used in clinical situations where myocardial ischemia/ reperfusion (I/R) injury is a threat. The aim of the present study was to evaluate the protective effect of propofol on myocardial I/R injury in rat due to apoptosis. Myocardial I/R injury were induced by occluding the left anterior descending (LAD) coronary artery for 25 min followed by either 2 h or 6 h reperfusion. Apoptosis was evaluated by Western blot analysis (Bcl-2, Bax expression), DNA strand breaks, TUNEL analysis and measuring myocardial caspase-3 activity. Propofol significantly reduced infarct size and improved I/R-induced myocardial contractile dysfunction by improving left ventricular diastolic pressure and positive and negative maximal values of the first derivative (+dp/dt) of left ventricular pressure. Propofol increased Bcl-2/Bax expression ratio and decreased caspase-3 activity in I/R rat hearts, which resulted in reduction of myocardial apoptosis as evidenced by TUNEL analysis and DNA laddering experiments. In an in vitro study, propofol increased H9c2 cell viability against oxidative stress induced by glucose oxidase (GOX) in a dose-dependent manner. These data suggest propofol limits I/R injury with an associated reduction in apoptotic cell death in vivo. |
| | |
Authors:
|
Yong Chun Jin; Wooyeol Kim; Yu Mi Ha; Il Woo Shin; Ju Tae Sohn; Hye Jung Kim; Han Geuk Seo; Jea Heun Lee; Ki Churl Chang |
Related Documents
:
|
6742324 - Blunt cardiac trauma. 8461514 - Myocardial stunning and preconditioning: age, species, and model related differences: r... 15280094 - Lymphatic diversion prevents myocardial edema following mesenteric ischemia/reperfusion. 1810434 - Cardioprotective effects of sodium gamma-hydroxybutyrate (ghb) on brain induced myocard... 477344 - Recovery from alexia without agraphia: report of an autopsy. 7994824 - Radiofrequency ablation of atrial flutter. efficacy of an anatomically guided approach. |
Publication Detail:
|
Type: Journal Article Date: 2008-10-28 |
Journal Detail:
|
Title: Vascular pharmacology Volume: 50 ISSN: 1537-1891 ISO Abbreviation: Vascul. Pharmacol. Publication Date: 2009 Jan-Feb |
Date Detail:
|
Created Date: 2008-12-16 Completed Date: 2009-04-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101130615 Medline TA: Vascul Pharmacol Country: United States |
Other Details:
|
Languages: eng Pagination: 71-7 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anesthetics, Intravenous
/
pharmacology Animals Apoptosis / drug effects* Caspase 3 / analysis Hypnotics and Sedatives / pharmacology In Situ Nick-End Labeling Male Myocardial Contraction / drug effects Myocardial Reperfusion Injury / drug therapy*, pathology, physiopathology Myocytes, Cardiac / drug effects, metabolism, pathology Propofol / pharmacology* Rats Rats, Sprague-Dawley bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anesthetics, Intravenous; 0/Hypnotics and Sedatives; 0/bcl-2-Associated X Protein; 2078-54-8/Propofol; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of polar membrane lipids of the extremely halophilic bacterium Salinibacter ruber a...
Next Document: Effects of testosterone therapy on muscle performance and physical function in older men with mobili...