| Propidium iodide for making heterochromatin more evident in the C-banding technique. | |
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MedLine Citation:
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PMID: 22747174 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light. |
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Authors:
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Rl Lui; Dr Blanco; O Moreira-Filho; Vp Margarido |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-2 |
Journal Detail:
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Title: Biotechnic & histochemistry : official publication of the Biological Stain Commission Volume: - ISSN: 1473-7760 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107378 Medline TA: Biotech Histochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Genetics and Evolution, Federal University of São Carlos , Highway Washington Luís (SP 310) Km 235, CEP: 13565-905 , São Carlos. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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