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Propidium iodide for making heterochromatin more evident in the C-banding technique.
MedLine Citation:
PMID:  22747174     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light.
Authors:
Rl Lui; Dr Blanco; O Moreira-Filho; Vp Margarido
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-2
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  -     ISSN:  1473-7760     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Genetics and Evolution, Federal University of São Carlos , Highway Washington Luís (SP 310) Km 235, CEP: 13565-905 , São Carlos.
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