Document Detail


Prophylactic, therapeutic and neutralizing effects of zinc oxide tetrapod structures against herpes simplex virus type-2 infection.
MedLine Citation:
PMID:  23047013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The attachment of Herpes simplex virus type-2 (HSV-2) to a target cell requires ionic interactions between negatively charged cell surface co-receptor heparan sulfate (HS) and positively charged residues on viral envelop glycoproteins, gB and gC. Effective blocking of this first step of HSV-2 pathogenesis demonstrates significant prophylactic effects against the viral disease; any in vitro therapeutic effects of blocking this interaction, however, are not clear. Here, we provide new evidence that zinc oxide tetrapod micro-nanostructures synthesized by flame transport approach significantly block HSV-2 entry into target cells and, in addition, demonstrate the potential to stop the spread of the virus among already infected cells. The zinc oxide tetrapods (ZnOTs) also exhibit the ability to neutralize HSV-2 virions. Natural target cells such as human vaginal epithelial and HeLa cells showed highly reduced infectivity when infected with HSV-2 virions that were pre-incubated with the ZnOTs. The mechanism behind the ability of ZnOTs to prevent, neutralize or reduce HSV-2 infection relies on their ability to bind the HSV-2 virions. We used fluorescently labeled ZnOTs and GFP-expressing HSV-2 virions to demonstrate the binding of the ZnOTs with HSV-2. We also show that the binding and hence, the antiviral effects of ZnOTs can be enhanced by illuminating the ZnOTs with UV light. Our results provide new insights into the anti-HSV-2 effects of ZnOT and rationalize their development as a HSV-2 trapping agent for the prevention and/or treatment of infection. The observed results also demonstrate that blocking HSV-2 attachment can have prophylactic as well as therapeutic applications.
Authors:
Thessicar E Antoine; Yogendra K Mishra; James Trigilio; Vaibhav Tiwari; Rainer Adelung; Deepak Shukla
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-06
Journal Detail:
Title:  Antiviral research     Volume:  96     ISSN:  1872-9096     ISO Abbreviation:  Antiviral Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2013-05-16     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  8109699     Medline TA:  Antiviral Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  363-75     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiviral Agents / pharmacology*
CHO Cells
Cell Survival
Cricetinae
Epithelial Cells / cytology,  virology
Female
Green Fluorescent Proteins / metabolism
HeLa Cells
Herpes Simplex / drug therapy*,  prevention & control
Herpesvirus 2, Human / drug effects*,  pathogenicity,  physiology
Humans
Microbial Sensitivity Tests
Nanostructures
Neutralization Tests
Vagina / cytology
Viral Plaque Assay
Virus Attachment
Virus Internalization / drug effects*
Zinc Oxide / pharmacology*
Grant Support
ID/Acronym/Agency:
AI081869/AI/NIAID NIH HHS; EY01792/EY/NEI NIH HHS; K02 AI081869/AI/NIAID NIH HHS; P30 EY001792/EY/NEI NIH HHS; R01 AI057860/AI/NIAID NIH HHS; R01 AI057860/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antiviral Agents; 147336-22-9/Green Fluorescent Proteins; SOI2LOH54Z/Zinc Oxide
Comments/Corrections

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