Document Detail


Properties of a variant of Streptococcus mutans altered in its ability to interact with glucans.
MedLine Citation:
PMID:  863516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After continuous subculture of Streptococcus mutans GS-5, the resultant organism, GS-5var, was found to be altered in its ability to undergo dextran- and sucrose-induced cell agglutination and to adhere to smooth surfaces in the presence of sucrose. Both GS-5 and GS-5var cells possessed the serotype c polysaccharide antigen, displayed similar growth kinetics, appeared to have similar metabolic and biosynthetic capabilities, and produced similar amounts of cell-associated and extracellular glucosyltransferase activity in chemically defined medium. GS-5 cells which had bound exogenous glucosyltransferase enzymes from either cell type were able to undergo sucrose-dependent adherence to smooth surfaces, whereas GS-5var cells which had bound comparable levels of glucosyltransferase activity were unable to adhere. In contrast to GS-5var cells, GS-5 cells were also able to adhere to preformed glucan deposits. In addition, [(14)C]glucan binding studies demonstrated that GS-5 cells were able to bind fourfold-greater levels of [(14)C]glucan relative to GS-5var cells having similar cell-associated glucosyltransferase activity. Further study of GS-5 cells also suggested the existence of a glucan binding site, distinct from the cell-associated glucosyltransferase activity, which is important in dextran-induced agglutination and sucrose-dependent adherence to smooth surfaces. These results are discussed in terms of the alteration of the GS-5var cells and their possible relationship to the putative receptors for glucosyltransferase enzymes and glucans on the cell surface of S. mutans.
Authors:
W M Janda; H K Kuramitsu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  16     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-07-29     Completed Date:  1977-07-29     Revised Date:  2010-09-03    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  575-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests
Antigens, Bacterial
Binding Sites
Culture Media
Genetic Variation*
Glucose / analogs & derivatives*
Hot Temperature
Polysaccharides / metabolism*
Pronase / pharmacology
Serotyping
Streptococcus / metabolism*
Streptococcus mutans / growth & development,  metabolism*
Transferases / metabolism
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Culture Media; 0/Polysaccharides; 50-99-7/Glucose; EC 2.-/Transferases; EC 3.4.24.-/Pronase
Comments/Corrections

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