| Properties of a variant of Streptococcus mutans altered in its ability to interact with glucans. | |
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MedLine Citation:
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PMID: 863516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After continuous subculture of Streptococcus mutans GS-5, the resultant organism, GS-5var, was found to be altered in its ability to undergo dextran- and sucrose-induced cell agglutination and to adhere to smooth surfaces in the presence of sucrose. Both GS-5 and GS-5var cells possessed the serotype c polysaccharide antigen, displayed similar growth kinetics, appeared to have similar metabolic and biosynthetic capabilities, and produced similar amounts of cell-associated and extracellular glucosyltransferase activity in chemically defined medium. GS-5 cells which had bound exogenous glucosyltransferase enzymes from either cell type were able to undergo sucrose-dependent adherence to smooth surfaces, whereas GS-5var cells which had bound comparable levels of glucosyltransferase activity were unable to adhere. In contrast to GS-5var cells, GS-5 cells were also able to adhere to preformed glucan deposits. In addition, [(14)C]glucan binding studies demonstrated that GS-5 cells were able to bind fourfold-greater levels of [(14)C]glucan relative to GS-5var cells having similar cell-associated glucosyltransferase activity. Further study of GS-5 cells also suggested the existence of a glucan binding site, distinct from the cell-associated glucosyltransferase activity, which is important in dextran-induced agglutination and sucrose-dependent adherence to smooth surfaces. These results are discussed in terms of the alteration of the GS-5var cells and their possible relationship to the putative receptors for glucosyltransferase enzymes and glucans on the cell surface of S. mutans. |
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Authors:
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W M Janda; H K Kuramitsu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 16 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1977 May |
Date Detail:
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Created Date: 1977-07-29 Completed Date: 1977-07-29 Revised Date: 2010-09-03 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 575-86 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agglutination Tests Antigens, Bacterial Binding Sites Culture Media Genetic Variation* Glucose / analogs & derivatives* Hot Temperature Polysaccharides / metabolism* Pronase / pharmacology Serotyping Streptococcus / metabolism* Streptococcus mutans / growth & development, metabolism* Transferases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Culture Media; 0/Polysaccharides; 50-99-7/Glucose; EC 2.-/Transferases; EC 3.4.24.-/Pronase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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