| Properties of proteases from milk somatic cells and blood leukocytes. | |
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MedLine Citation:
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PMID: 1894806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proteolytic activity of proteases associated with somatic cells isolated from high SCC milk and proteases associated with leukocytes isolated from bovine blood was assayed at pH 6.6 and 5.2 in a model system consisting of a beta-casein (.96%) substrate in Jenness/Koops buffer. Intact beta-casein and casein proteolysis products were measured by densitometric analysis of SDS-PAGE gels. Relative proteolytic activity was expressed as percentage degradation of beta-casein after 24 h at 37 degrees C per 1 x 10(6) cells/ml. Proteolytic activity associated with somatic cells isolated from bovine milk was 27.5 and 13.6% at pH 6.6 and 5.2, respectively. Proteolytic activity associated with leukocytes isolated from bovine blood was 16.0 and 8.4% at pH 6.6 and 5.2, respectively. Proteolytic activity at pH 6.6 was significantly higher for the somatic cells isolated from milk than for leukocytes isolated from blood. The reason for the difference in proteolytic activity of leukocytes isolated from blood of a healthy cow versus somatic cells isolated from milk produced by a cow with mastitis is not known. Further work is needed to determine whether the difference may be caused by a higher proportion of activated macrophages in somatic cells isolated from milk produced by cows with mastitis than in the leukocytes isolated from blood of healthy cows. |
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Authors:
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R J Verdi; D M Barbano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 74 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 1991 Jul |
Date Detail:
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Created Date: 1991-10-24 Completed Date: 1991-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2077-81 Citation Subset: IM |
Affiliation:
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Department of Food Science, Cornell University, Ithaca, NY 14853. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caseins / metabolism Cattle Cell Count / veterinary Densitometry Electrophoresis, Polyacrylamide Gel Endopeptidases / blood, metabolism* Female Hydrogen-Ion Concentration Leukocytes / enzymology* Macrophages / enzymology* Mastitis, Bovine / blood, enzymology* Milk / cytology, enzymology* Neutrophils / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Caseins; EC 3.4.-/Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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