Document Detail


Properties of proteases from milk somatic cells and blood leukocytes.
MedLine Citation:
PMID:  1894806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteolytic activity of proteases associated with somatic cells isolated from high SCC milk and proteases associated with leukocytes isolated from bovine blood was assayed at pH 6.6 and 5.2 in a model system consisting of a beta-casein (.96%) substrate in Jenness/Koops buffer. Intact beta-casein and casein proteolysis products were measured by densitometric analysis of SDS-PAGE gels. Relative proteolytic activity was expressed as percentage degradation of beta-casein after 24 h at 37 degrees C per 1 x 10(6) cells/ml. Proteolytic activity associated with somatic cells isolated from bovine milk was 27.5 and 13.6% at pH 6.6 and 5.2, respectively. Proteolytic activity associated with leukocytes isolated from bovine blood was 16.0 and 8.4% at pH 6.6 and 5.2, respectively. Proteolytic activity at pH 6.6 was significantly higher for the somatic cells isolated from milk than for leukocytes isolated from blood. The reason for the difference in proteolytic activity of leukocytes isolated from blood of a healthy cow versus somatic cells isolated from milk produced by a cow with mastitis is not known. Further work is needed to determine whether the difference may be caused by a higher proportion of activated macrophages in somatic cells isolated from milk produced by cows with mastitis than in the leukocytes isolated from blood of healthy cows.
Authors:
R J Verdi; D M Barbano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  74     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-10-24     Completed Date:  1991-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2077-81     Citation Subset:  IM    
Affiliation:
Department of Food Science, Cornell University, Ithaca, NY 14853.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caseins / metabolism
Cattle
Cell Count / veterinary
Densitometry
Electrophoresis, Polyacrylamide Gel
Endopeptidases / blood,  metabolism*
Female
Hydrogen-Ion Concentration
Leukocytes / enzymology*
Macrophages / enzymology*
Mastitis, Bovine / blood,  enzymology*
Milk / cytology,  enzymology*
Neutrophils / enzymology
Chemical
Reg. No./Substance:
0/Caseins; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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