| Properties of a polygalacturonase-inhibiting protein isolated from 'Oroblanco' grapefruit. | |
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MedLine Citation:
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PMID: 15032836 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Polygalacturonase inhibiting protein (PGIP) was extracted from 'Oroblanco' grapefruit type (triploid pummelo-grapefruit) albedo tissue, purified and partially characterized. Extraction was carried out at 4 degrees C with a high ionic strength extraction buffer. After dialysis and concentration by ultrafiltration the extract was chromatographed on concanavalin A-Sepharose. The PGIP activity was bound by the lectin and then eluted using 250 mM alpha-methyl mannopyranoside, resulting in a 17-fold purification of the PGIP and demonstrating its glycoprotein nature. The anion-exchange and size-exclusion chromatography steps that followed gave a PGIP that was 857-fold purified relative to the initial tissue extract, and having a 44 kDa molecular weight, as estimated by SDS-PAGE electrophoresis. PGIP inhibition activity was tested with endo-polygalacturonase (EC 3.2.1.15) produced by Penicillium italicum and Botrytis cinerea. The radial diffusion and reducing sugar assays showed that P. italicum and B. cinerea endo-PGs were affected by PGIP, whereas no endo-PG activity was detected in the culture filtrate of P. digitatum. In vitro tests revealed that PGIP inhibited P. italicum and B. cinerea growth. By contrast, the influence of PGIP on P. digitatum, growth was negligible, perhaps because this fungus does not produce endo-PG. Following heating for 10 min at 65 degrees C the inhibitory activity of PGIP was reduced by 43%. PGIP activity decreased further as heating temperature increased, and was completely suppressed after heating at 100 degrees C for 10 min. |
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Authors:
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Guy D'hallewin; Mario Schirra; Ann L. T. Powell; L. Carl Greve; John M. Labavitch |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Physiologia plantarum Volume: 120 ISSN: 1399-3054 ISO Abbreviation: Physiol Plant Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-Mar-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1256322 Medline TA: Physiol Plant Country: - |
Other Details:
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Languages: ENG Pagination: 395-404 Citation Subset: - |
Affiliation:
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C.N.R. - Istituto di Scienze delle Produzioni Alimentari, Sezione di Sassari, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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