Document Detail

Properties of multinucleated giant cells in a new in vitro model for human granuloma formation.
MedLine Citation:
PMID:  9227348     Owner:  NLM     Status:  MEDLINE    
Multinucleated giant cells (MGCs) are a key feature of granulomas. They have been studied with respect to the mechanism and regulation of their formation, but the function of these cells still remains elusive. A new method for the in vitro generation of granulomas was developed and characterized in which L3 larvae of Nippostrongylus brasiliensis, as a target for the cellular response, were co-incubated with human mononuclear blood cells. The development of epithelioid cells and MGCs was observed and single isolated MGCs were analysed by the reverse transcriptase polymerase chain reaction method. The presence of tumour necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) transcripts in MGCs was demonstrated. It is proposed that MGCs in the granuloma model may in part represent an active cellular constituent involved in granuloma formation and turnover and in the destruction of the irritant.
U Seitzer; D Scheel-Toellner; K M Toellner; N Reiling; H Haas; J Galle; H D Flad; J Gerdes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  182     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-01     Completed Date:  1997-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
Department of Immunology and Cell Biology, Forschungszentrum Borstel, Germany.
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MeSH Terms
Cell Culture Techniques
Cytokines / genetics,  metabolism
Giant Cells / immunology*,  ultrastructure
Granuloma / immunology*,  metabolism,  pathology
Immunoenzyme Techniques
Models, Biological*
Polymerase Chain Reaction
Rats, Wistar
Reg. No./Substance:

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