| Properties of intracellular ribonuclease utilized for RNA reduction in disintegrated cells of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 15672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The properties of intracellular RNase in disintegrated cell suspensions of Saccharomyces cerevisiae have been studied. The influence of salt addition and/or incubation of the suspension on the activity of RNase and on the degradation of endogenous RNA was determined. No significant change in the RNase activity in the disintegrated suspensions was obtained by addition of 3% NaCl or by incubation at 50 degrees C with 3% NaCl. During the incubation with NaCl the active RNase was able to degrade endogenous RNA. By incubation without salt the RNase was inactivated. Inactivation also occurred after extraction at alkaline pH. The RNase had an optima at pH 5-6 and temperatures between 50-60 degrees C. The main part of the RNase in the unincubated suspension was soluble also at pH 4.0. No serious protein degradation occurred during the short time incubation needed for RNA reduction. 70% of the protein in the suspensions was recovered in the precipitate at pH 4.0 after 20 min of incubation. The corresponding protein recovery from unincubated suspensions was 77%. |
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Authors:
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M Lindblom |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 19 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1977 Feb |
Date Detail:
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Created Date: 1977-06-11 Completed Date: 1977-06-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 199-210 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzyme Activation
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drug effects Fungal Proteins / isolation & purification* Hot Temperature Hydrogen-Ion Concentration Ribonucleases / metabolism* Saccharomyces cerevisiae / enzymology* Sodium Chloride / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 7647-14-5/Sodium Chloride; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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