Document Detail

Properties of human thymic B cells.
MedLine Citation:
PMID:  1592434     Owner:  NLM     Status:  MEDLINE    
B cells, distinct from those seen in myasthenia gravis, are present in normal human thymic medulla, concentrated around the Hassall's corpuscles. We have shown that they constitute 33 +/- 4.8% of the total cells in the thymic medulla. In tissue sections they were often seen to have rosettes of thymocytes around them, a relationship which was maintained when the cells were isolated from the thymus. Thymic B cells expressed cytoplasmic immunoglobulins IgD, IgM and IgG but only rarely IgA. Unlike murine thymic B cells, human thymic B cells were CD5-. Freshly isolated thymic B cells were activated cells, but they rapidly became quiescent and died in culture over a 10-day period unless stimulated with mitogens. Thymic B cells responded to polyclonal B-cell activators SAC and TPA and when stimulated, maintained their relationship with thymocytes. Electron microscopic studies showed that two morphologically different thymocyte populations associated with the B cells. The plasma membranes of larger thymocytes were juxtaposed to the B-cell membrane, but smaller thymocytes with darker cytoplasm were associated with the B cells via cytoplasmic strands. Studies in mice have suggested that B cells are involved in thymic negative selection. The close association between activated B cells and thymocytes observed in this study supports this hypothesis.
J Spencer; M Choy; T Hussell; L Papadaki; J P Kington; P G Isaacson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  75     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-07-02     Completed Date:  1992-07-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  596-600     Citation Subset:  IM    
Dept. of Histopathology, UCMSM, University St, London, U.K.
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MeSH Terms
B-Lymphocytes / immunology*,  ultrastructure
Child, Preschool
Immunoenzyme Techniques
Immunoglobulin D / analysis
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Lymphocyte Activation / immunology
Mitogens / immunology
T-Lymphocytes / ultrastructure
Thymus Gland / immunology*,  ultrastructure
Reg. No./Substance:
0/Immunoglobulin D; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Mitogens

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