| Properties of the histones and functional aspects of the soluble chromatin of epimastigote Trypanosoma cruzi. | |
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MedLine Citation:
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PMID: 8907392 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amino acid composition of all histones of Trypanosoma cruzi was analyzed, and the terminology of the histones of higher eukaryotes adopted. One chromatin associated protein, previously considered to be a variant of histone H1, could not be clearly identified, and shows features of core histones as well as of histone H1. An improved method for the isolation of intact nuclei and the production of soluble chromatin in T. cruzi was established. The chromatin of T. cruzi is relatively instable and histone H1 is easily lost during experimental manipulations. Histone H1 dissociates completely at a relatively low NaCl concentration of 380 mM, leading to an open nucleosome filament which does not condense. The influence of histone H1 of T. cruzi and of rat liver on the compaction pattern of the chromatin was investigated by homologous and heterologous reconstitution experiments, and analysed by electron microscopy. It could be shown that histone H1 of T. cruzi induces nucleosome filaments of T. cruzi as well as those of rat liver to condense. The same is true for histone H1 of rats. It can be concluded that T. cruzi has a functional histone H1. |
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Authors:
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W Schlimme; M Burri; B Betschart; H Hecker |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta tropica Volume: 60 ISSN: 0001-706X ISO Abbreviation: Acta Trop. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1997-02-13 Completed Date: 1997-02-13 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370374 Medline TA: Acta Trop Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 141-54 Citation Subset: IM |
Affiliation:
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Swiss Tropical Institute, Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chromatin / chemistry*, physiology Histones / analysis* Nucleosomes / chemistry Rats Trypanosoma cruzi / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Chromatin; 0/Histones; 0/Nucleosomes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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